Journal of Biological Chemistry
Protein Structure and FoldingLanthanide-dependent alcohol dehydrogenases require an essential aspartate residue for metal coordination and enzymatic function
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This article contains supporting information.
Author contributions—N. M. G. and N. C. M.-G. conceptualization; N. M. G. and M. F. data curation; N. M. G., M. F., J. H., R. P. H., and N. C. M.-G. formal analysis; N. M. G., J. H., R. P. H., and N. C. M.-G. supervision; N. M. G. and M. F. validation; N. M. G., M. F., and K. D. investigation; N. M. G. and M. F. visualization; N. M. G. and M. F. methodology; N. M. G. writing-original draft; N. M. G., M. F., K. D., J. H., R. P. H., and N. C. M.-G. writing-review and editing; M. F., J. H., R. P. H., and N. C. M.-G. funding acquisition; J. H., R. P. H., and N. C. M.-G. project administration.
Funding and additional information—This material is based upon work supported by the National Science Foundation under Grants 1750003 (to N. C. M.-G. and N. M. G.) and CHE-1516126 (to R. P. H. and J. H.). M. F. was supported by University of Otago Health Sciences Postdoctoral Fellowship HSCDPD1703.
Conflict of interest—The authors declare that they have no conflicts of interest with the contents of this article.
Abbreviations—The abbreviations used are:
- PQQ
pyrroloquinoline quinone
- DFT
density functional theory
- MDH
methanol dehydrogenase
- EDH
ethanol dehydrogenase
- ADH
alcohol dehydrogenase
- Ln-ADH
Ln3+-dependent alcohol dehydrogenase
- IMAC
immobilized metal affinity chromatography
- RMSD
root mean square deviation
- PDB
Protein Data Bank
- ANOVA
analysis of variance
- PMS
phenazine methosulfate
- DCPIP
2,6-dichlorophenol indophenol
- ICP
inductively coupled plasma
- OES
optical emission spectroscopy
- OD
optical density
- RMSE
root mean square error
- TEV
tobacco etch virus.
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These authors contributed equally to this work.