Journal of Biological Chemistry
EnzymologyA carbohydrate-binding family 48 module enables feruloyl esterase action on polymeric arabinoxylanArabinoxylan-binding CBM48
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This work was supported by Villum Foundation Grant VKR022796 (to L. L.) and base funding from the Technical University of Denmark. J. B. and K. B. R. M. K. are employees at Novozymes A/S, a company that produces and sells enzymes and microbes for industrial uses.
This article contains Figs. S1–S9 and Table S1.
The protein sequence for the wtsFae1B gene has been deposited in the NCBI Protein Database under NCBI accession no.BK010854.1.
The atomic coordinates and structure factors (codes 6RZO and 6RZN) have been deposited in the Protein Data Bank (http://wwpdb.org/).
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Supported by Novo Nordisk Foundation Grant NNF10CC1016517 to the Novo Nordisk Foundation Center for Biosustainability at Technical University of Denmark.
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Supported by The Independent Research Fund Denmark, Natural Sciences Grant 6108-00476A.
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The abbreviations used are:
- AX
arabinoxylans
- Araf
arabinofuranose
- AXOS
arabinoxylooligosaccharide
- CAZy
Carbohydrate-Active EnZyme database
- CBM
carbohydrate-binding module
- CBM48
carbohydrate-binding module family 48
- CE1
carbohydrate esterase family 1
- di-FA
ferulate dehydrodimer
- FA
ferulic acid
- GH10
glycoside hydrolase family 10
- r.m.s.d.
root-mean-square deviation
- SPR
surface plasmon resonance
- WAX-I
insoluble wheat arabinoxylan
- Xylp
xylopyranose
- XA5f2X
β-d-Xylp-(1→4)-[5-O-feruloyl-α-l-Araf-(1→3)]-β-d-Xylp-(1→4)-β-d-Xylp
- Bis-Tris
2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol
- Bistris propane
1,3-bis[tris(hydroxymethyl)methylamino]propane
- RU
resonance unit
- wts
wastewater treatment sludge
- DSC
differential scanning calorimetry.