Journal of Biological Chemistry
Volume 288, Issue 46, 15 November 2013, Pages 33303-33311
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Cell Biology
Mitochondrial Dysfunction and Permeability Transition in Osteosarcoma Cells Showing the Warburg Effect*

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Metabolic reprogramming in cancer is manifested by persistent aerobic glycolysis and suppression of mitochondrial function and is known as the Warburg effect. The Warburg effect contributes to cancer progression and is considered to be a promising therapeutic target. Understanding the mechanisms used by cancer cells to suppress their mitochondria may lead to development of new approaches to reverse metabolic reprogramming. We have evaluated mitochondrial function and morphology in poorly respiring LM7 and 143B osteosarcoma (OS) cell lines showing the Warburg effect in comparison with actively respiring Saos2 and HOS OS cells and noncancerous osteoblastic hFOB cells. In LM7 and 143B cells, we detected markers of the mitochondrial permeability transition (MPT), such as mitochondrial swelling, depolarization, and membrane permeabilization. In addition, we detected mitochondrial swelling in human OS xenografts in mice and archival human OS specimens using electron microscopy. The MPT inhibitor sanglifehrin A reversed MPT markers and increased respiration in LM7 and 143B cells. Our data suggest that the MPT may play a role in suppression of mitochondrial function, contributing to the Warburg effect in cancer.

Cancer
Glycolysis
Mitochondria
Mitochondrial Permeability Transition
Osteosarcoma
Warburg Effect
Cyclophilin D

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*

This work was supported, in whole or in part, by National Institutes of Health Grant KL2 RR024136 from the National Center for Research Resources and Grants R03 AR061515 and P30 AR061307 from NIAMS. This work was also supported by the Center for Musculoskeletal Research, BeatSarcoma, and the Karen D'Amico Foundation.