Journal of Biological Chemistry
Volume 275, Issue 31, 4 August 2000, Pages 23685-23692
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PROTEIN SYNTHESIS POST-TRANSLATION MODIFICATION AND DEGRADATION
Endoplasmic Reticulum Oxidoreductin 1-Lβ (ERO1-Lβ), a Human Gene Induced in the Course of the Unfolded Protein Response*210

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Oxidative conditions must be generated in the endoplasmic reticulum (ER) to allow disulfide bond formation in secretory proteins. A family of conserved genes, termed EROfor ER oxidoreductins, plays a key role in this process. We have previously described the human gene ERO1-L, which complements several phenotypic traits of the yeast thermo-sensitive mutant ero1-1 (Cabibbo, A., Pagani, M., Fabbri, M., Rocchi, M., Farmery, M. R., Bulleid, N. J., and Sitia, R. (2000)J. Biol. Chem. 275, 4827–4833). Here, we report the cloning and characterization of a novel human member of this family,ERO1-Lβ. Immunofluorescence, endoglycosidase sensitivity, and in vitro translation/translocation assays reveal that the products of the ERO1-Lβ gene are primarily localized in the ER of mammalian cells. The ability to allow growth at 37 °C and to alleviate the “unfolded protein response” when expressed in ero1-1 cells indicates that ERO1-Lβ is involved also in generating oxidative conditions in the ER. ERO1-L and ERO1-Lβ display different tissue distributions. Furthermore, only ERO1-Lβ transcripts are induced in the course of the unfolded protein response. Our results suggest a complex regulation of ER redox homeostasis in mammalian cells.

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Published, JBC Papers in Press, May 18, 2000, DOI 10.1074/jbc.M003061200

*

This work was supported in part through grants from Associazione per la Ricerca sul Cancro, Consiglio Nazionale delle Ricerche (Target Project on Biotechnology, Grant CNR 97.01296.PF49; 5% Biotechnology, Grant CNR 98.00393.PF31), Ministero della Sanità (RF 9853), and Biotechnology and Biological Sciences Research Council (Grant 34/C09198).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank™/EMBL Data Bank with accession number(s).

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The on-line version of this article (available athttp://www.jbc.org) contains supplementary quantitative data on the tissue distribution of ER01-Lα, ER01-Lβ, and ubiquitinobtained by PhosphorImager (Molecular Probes). The ratios between the different transcripts in the various tissues are also reported.

§

These authors contributed equally to this work.

Present address: Dept. of Applied and Molecular Ecology, Waite Campus, Glen Osmond, South Australia 5064, Australia.