Skip to main content
SpringerLink
Log in

Molecular variability and mating-type frequency of Ascochyta rabiei of chickpea from Saskatchewan, Canada

  • Published:
Australasian Plant Pathology Aims and scope Submit manuscript

Abstract

Molecular variability of populations of Ascochyta rabiei from Saskatchewan collected in 1998 and 2002, and mating-type frequency of isolates from 1998, 2001 and 2002 were assessed. To evaluate the genetic diversity of different A. rabiei populations, 30 isolates from 1998 and 30 isolates from 2002 were compared by random amplified polymorphic DNA fingerprinting. Cluster analysis and analysis of molecular variance suggested differentiation of the 1998 and 2002 populations, yet equal amplicon diversity between populations with the majority of the variation occurring within each population was observed. Analysis of mating-type frequencies on 30 isolates each from 1998, 2001 and 2002 populations did not significantly depart from a 1 : 1 ratio suggesting random mating of each population. However, when 121 isolates from 2002 were analysed for mating type, a significant departure from a 1 : 1 ratio was found suggesting smaller sampling sizes for mating-type frequenciesmay not reveal true differences. In further support of the hypothesis of a randomly mating population in 1998, the Index of Association was not significantly different from zero. However, this measure suggested departure from panamixa in the 2002 population consistent with the skewed mating-type ratio. It was concluded that there was some evidence for genetic shift in populations of A. rabiei between 1998 and 2002, which is consistent with a shift in aggressiveness observed between these populations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Armstrong CL, Chongo G, Gossen BD, Duczek LJ (2001) Mating type distribution and incidence of the teleomorph of Ascochyta rabiei (Didymella rabiei) in Canada. Canadian Journal of Plant Pathology 23, 110–113.

    Article  Google Scholar 

  • Barve MP, Santra DK, Ranjekar PK, Gupta VS (2004) Genetic diversity analysis of a world-wide collection of Ascochyta rabiei isolates using sequence tagged microsatellite markers. World Journal of Microbiology & Biotechnology 20, 735–741. doi: 10.1007/s11274-004-1550-8

    Article  CAS  Google Scholar 

  • Barve MP, Arie T, Salimath SS, Muehlbauer FJ, Peever TL (2003) Cloning and characterization of the mating type (MAT) locus from Ascochyta rabiei (teleomorph: Didymella rabiei) and a MAT phylogeny of legumeassociated Ascochyta spp. Fungal Genetics and Biology 39, 151–167. doi: 10.1016/S1087-1845(03)00015-X

    Article  CAS  PubMed  Google Scholar 

  • Chongo G, Gossen BD (2001) Effect of plant age on resistance to Ascochyta rabiei in chickpea. Canadian Journal of Plant Pathology 23, 358–363.

    Article  Google Scholar 

  • Chongo G, Gossen BD, Buchwaldt L, Adhikari T, Rimmer SR(2004) Genetic diversity of Ascochyta rabiei in Canada. Plant Disease 88, 4–10. doi: 10.1094/PDIS.2004.88.1.4

    Article  Google Scholar 

  • Coppin E, Debuchy R, Arnaise S, Picard M (1997) Mating types and sexual development in filamentous ascomycetes. Microbiology and Molecular Biology Reviews 61, 411–428.

    CAS  PubMed  Google Scholar 

  • Dice LR (1945) Measures of the amount of ecology association between species. Ecology 26, 297–302. doi: 10.2307/1932409

    Article  Google Scholar 

  • Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes — application to human mitochondrial-DNA restriction data. Genetics 131, 479–491.

    CAS  PubMed  Google Scholar 

  • Galloway J, MacLeod WJ (2003) Didymella rabiei, the teleomorph of Ascochyta rabiei, found on chickpea stubble in Western Australia. Australasian Plant Pathology 32, 127–128. doi: 10.1071/AP02076

    Article  Google Scholar 

  • Geistlinger J, Maqbool S, Kaiser WJ, Kahl G (1997a) Detection of microsatellite fingerprint markers and their Mendelian inheritance in Ascochyta rabiei. Mycological Research 101, 1113–1121. doi: 10.1017/S0953756297004231

    Article  CAS  Google Scholar 

  • Geistlinger J, Weising K, Kaiser WJ, Kahl G (1997b) Allelic variation at a hypervariable compound microsatellite locus in the ascomycete Ascochyta rabiei. Molecular & General Genetics 256, 298–305. doi: 10.1007/s004380050573

    Article  CAS  Google Scholar 

  • Geistlinger J, Weising K, Winter P, Kahl G (2000) Locus-specific microsatellite markers for the fungal chickpea pathogen Didymella rabiei (anamorph) Ascochyta rabiei. Molecular Ecology 9, 1939–1941. doi: 10.1046/j.1365-294x.2000.01092-13.x

    Article  CAS  PubMed  Google Scholar 

  • Jamil FF, Sarwar N, Sarwar M, Khan JA, Geistlinger J, Kahl G (2000) Genetic and pathogenic diversity within Ascochyta rabiei (Pass.) Lab. populations in Pakistan causing blight of chickpea (Cicer arietinum L.). Physiological and Molecular Plant Pathology 57, 243–254. doi: 10.1006/ pmpp.2000.0303

    Article  CAS  Google Scholar 

  • Kaiser WJ (1992) Epidemiology of Ascochyta rabiei. In ‘Disease resistance breeding in Kabuli chickpeas’. (Eds KB Singh, M Saxena) pp. 117–143. (ICARDA: Aleppo, Syria)

    Google Scholar 

  • Kaiser WJ (1995) World distribution of Didymella rabiei, the teleomorph of Ascochyta rabiei, on chickpea. Phytopathology 85, 1040.

    Google Scholar 

  • Kaiser WJ (1997) Inter- and international spread of ascochyta pathogens of chickpea, faba bean, and lentil. Canadian Journal of Plant Pathology 19, 215–224.

    Article  Google Scholar 

  • Kaiser WJ, Hannan RM (1987) First report of Mycosphaerella rabiei on chickpeas in the western-hemisphere. Plant Disease 71, 192. doi: 10.1094/PD-71-0192E

    Article  Google Scholar 

  • Kaiser WJ, Kusmenoglu I (1997) Distribution of mating types and the teleomorph of Ascochyta rabiei on chickpea in Turkey. Plant Disease 81, 1284–1287. doi: 10.1094/PDIS.1997.81.11.1284

    Article  Google Scholar 

  • Kaiser WJ, Okhovat M (1996) Distribution of Didymella rabiei, the teleomorph of Ascochyta rabiei, in Iran. Iranian Journal of Plant Pathology 32, 158–162.

    Google Scholar 

  • Khan MSA, Ramsey MD, Corbiere R, Infantino A, Porta-Puglia A, Bouznad Z, Scott ES (1999) Ascochyta blight of chickpea in Australia: identification, pathogenicity and mating type. Plant Pathology 48, 230–234. doi: 10.1046/j.1365-3059.1999.00338.x

    Article  Google Scholar 

  • Lichtenzveig J, Winter P, Abbo S, Shtienberg D, Kaiser WJ, Kahl G (2002) Towards the first linkage map of the Didymella rabiei genome. Phytoparasitica 30, 467–472. doi: 10.1007/BF02979751

    Article  CAS  Google Scholar 

  • Lichtenzveig J, Gamliel E, Frenkel O, Michaelido S, Abbo S, Sherman A, Shtienberg D (2005) Distribution of mating types and diversity in virulence of Didymella rabiei in Israel. European Journal of Plant Pathology 113, 15–24. doi: 10.1007/s10658-005-8914-2

    Article  Google Scholar 

  • Lougheed SC, Gibbs HL, Prior KA, Weatherhead PJ (2000) A comparison of RAPD versus microsatellite DNA markers in population studies of the Massasuga rattlesnake. Journal of Heredity 91, 458–463. doi: 10.1093/ jhered/91.6.458

    Article  CAS  PubMed  Google Scholar 

  • McDonald BA (1997) The population genetics of fungi: tools and techniques. Phytopathology 87, 448–453. doi: 10.1094/PHYTO.1997.87.4.448

    Article  CAS  PubMed  Google Scholar 

  • Metzenberg RL, Glass NL (1990) Mating type and mating strategies in Neurospora. BioEssays 12, 53–59. doi: 10.1002/bies.950120202

    Article  CAS  PubMed  Google Scholar 

  • Milgroom MG (1996) Recombination and the multilocus structure of fungal populations. Annual Review of Phytopathology 34, 457–477. doi: 10.1146/annurev.phyto.34.1.457

    Article  CAS  PubMed  Google Scholar 

  • Morjane H, Geistlinger J, Harrabi M, Weising K, Kahl G (1994) Oligonucleotide fingerprinting detects genetic diversity among Ascochyta rabiei isolates from a single chickpea field in Tunisia. Current Genetics 26, 191–197. doi: 10.1007/BF00309547

    Article  CAS  PubMed  Google Scholar 

  • Navas-Cortés JA, Perez-Artes E, Jimenez-Diaz RM, Llobell A, Bainbridge BW, Heale JB (1998) Mating type, pathotype and RAPDs analysis in Didymella rabiei, the agent of ascochyta blight of chickpea. Phytoparasitica 26, 199–212. doi: 10.1007/BF02981435

    Article  Google Scholar 

  • Nelson MA (1996) Mating systems in ascomycetes: a romp in the sac. Trends in Genetics 12, 69–74. doi: 10.1016/0168-9525(96)81403-X

    Article  CAS  PubMed  Google Scholar 

  • Peever TL, Salimath SS, Su G, Kaiser WJ, Muehlbauer FJ (2004) Historical and contemporary multilocus population structure of Ascochyta rabiei (teleomorph: Didymella rabiei) in the Pacific Northwest of the United States. Molecular Ecology 13, 291–309. doi: 10.1046/j.1365-294X.2003.02059.x

    Article  CAS  PubMed  Google Scholar 

  • Phan HTT, Ford R, Taylor PWJ (2003) Population structure of Ascochyta rabiei in Australia based on STMS fingerprints. Fungal Diversity 13, 111–129.

    Google Scholar 

  • Reynolds J, Weir BS, Cockerham CC (1983) Estimation of the co-ancestry coefficient — basis for a short-term genetic-distance. Genetics 105, 767–779.

    CAS  PubMed  Google Scholar 

  • Rohlf FJ (1994) ‘NTSYS-PC, numerical taxonomy and multivariate analysis system. Version 1.8.’ (Exeter Software: Setauket, NY)

    Google Scholar 

  • Santra DK, Singh G, Kaiser WJ, Gupta VS, Ranjekar PK, Muehlbauer FJ (2001) Molecular analysis of Ascochyta rabiei (Pass.) Labr., the pathogen of ascochyta blight in chickpea. Theoretical and Applied Genetics 102, 676–682. doi: 10.1007/s001220051696

    Article  CAS  Google Scholar 

  • Schneider S, Roessli D, Excoffier L (2000) Arlequin: a software for population genetics data analysis. Version 2.000. Department of Anthropology and Ecology, University of Geneva, Switzerland. Available at http://anthro. unige.ch/arlequin [Verified 19 February 2009]

    Google Scholar 

  • Shtienberg D, Vintal H, Nitzni Y, Brener S, Retig B (1998) The perfect stage of ascochyta blight (Didymella rabiei): a new threat to chickpea production in Israel? Phytoparasitica 26, 157.

    Google Scholar 

  • Sokal FJ, Rohlf RR (1981) ‘Biometry: the principles and practice of statistics in biological systems.’ 2nd edn. (W. H. Freeman and Company: New York)

    Google Scholar 

  • Trapero-Casas A, Navas-Cortés JA, Jimenez-Diaz RM (1996) Airborne ascospores of Didymella rabiei as a major primary inoculum for ascochyta blight epidemics in chickpea crops in southern Spain. European Journal of Plant Pathology 102, 237–245. doi: 10.1007/ BF01877962

    Article  Google Scholar 

  • Turgeon BG (1998) Application of mating type gene technology to problems in fungal biology. Annual Review of Phytopathology 36, 115–137. doi: 10.1146/annurev.phyto.36.1.115

    Article  CAS  PubMed  Google Scholar 

  • Turgeon BG, Bohlmann H, Ciuffetti LM, Christiansen SK,Yang G, Schafer W, Yoder OC (1993) Cloning and analysis of the mating-type genes from Cochliobolus heterostrophus. Molecular & General Genetics 238, 270–284.

    CAS  Google Scholar 

  • Udupa SM, Weigand F, Saxena MC, Kahl G (1998) Genotyping with RAPD and microsatellite markers resolves pathotype diversity in the ascochyta blight pathogen of chickpea. Theoretical and Applied Genetics 97, 299–307. doi: 10.1007/s001220050899

    Article  CAS  Google Scholar 

  • Vail S, Banniza S (2008) Structure and pathogenic variability in Ascochyta rabiei populations on chickpea in the Canadian prairies. Plant Pathology 57, 665–673. doi: 10.1111/j.1365-3059.2008.01837.x

    Article  Google Scholar 

  • Weising K, Kaemmer D, Epplen JT, Weigand F, Saxena M, Kahl G (1991) DNA fingerprinting of Ascochyta rabiei with synthetic oligodeoxynucleotides. Current Genetics 19, 483–489. doi: 10.1007/ BF00312740

    Article  CAS  Google Scholar 

  • Wilson AD, Kaiser WJ (1995) Cytology and genetics of sexual incompatibility in Didymella rabiei. Mycologia 87, 795–804. doi: 10.2307/3760856

    Article  Google Scholar 

  • Yates F (1934) Contingency table involving small numbers and the c2 test. Journal of the Royal Statistical Society (Supplement) 1, 217–235. doi: 10.2307/3760856

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Crop Development Centre, University of Saskatchewan, 51 Campus Drive, 5A8, Saskatoon, Saskatchewan S7N, Canada

    S. Vail & S. Banniza

Authors
  1. S. Vail
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Banniza
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. Vail.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vail, S., Banniza, S. Molecular variability and mating-type frequency of Ascochyta rabiei of chickpea from Saskatchewan, Canada. Australasian Plant Pathology 38, 392–398 (2009). https://doi.org/10.1071/AP09014

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1071/AP09014

Additional keywords

Search

Navigation