Society of University SurgeonsCD8+ T cells express a T-helper 1–like phenotype after burn injury*,**
Section snippets
Animals
C57BL/6 (H-2b)-transgenic mice (HY TCR)16 that carry the transgene specific for male HY antigen were obtained from the National Institute of Arthritis and Infectious Diseases through Taconic Laboratories (Germantown, NY).
Mouse burn injury
Fifteen-to 20-gm, 6-week-old, female HY TCR C57BL/6 mice were used as subjects in all experiments. All protocols were performed in accordance with the National Institutes of Health guidelines and approved by the University of North Carolina Committee on Animal Research. The
Effect of burn injury antigen-specific proliferation in HY TCR mice
To ensure that we had a measurable immunologic deficit in T-cell activity after burn injury, we chose 72 hours after the burn injury to kill the mice and to perform our antigen-specific stimulation experiments. In our previous studies, CTL activity is maximally depressed 72 hours after a 20% burn,18, 19 and we expected that the HY antigen–specific proliferative response in the HY TCR transgenic mouse model would be suppressed at this time point as well. For nearly all concentrations of HY
Discussion
In this study, we demonstrate that a 20% TBSA burn induces an antigen-specific, dysfunctional T-cell proliferative response 72 hours after injury in HY TCR transgenic mice. This immune dysfunction is associated with a TH1-like phenotype in activated CD8+ T cells from burned mice, with increased IFN-γ and IL-2 intracellular staining, compared with control mice. Furthermore, this TH1-like phenotype is sustained after prolonged antigen-specific stimulation in culture. Although the percentage of CD8
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Sex differences and estrogen modulation of the cellular immune response after injury
2008, Cellular ImmunologyCitation Excerpt :Consistent with this paradigm, some studies demonstrate a Th2 (Tc2) phenotype cytokine secretion by CD8+ T cells with a decreased antigen-specific proliferative and cytolytic response [77,78]. However, other groups have evidence of CD8+ T cells producing Th1 (Tc1) cytokines similar to their CD4+ T cell counterparts following burn [79]. To add confusion to the story, other laboratories suggest it is CD4+ CD25+ T regulatory cells that are the main players in down-regulating the aberrant inflammatory response following burn [80].
Transgenic and gene knock-out techniques and burn research
2005, Journal of Surgical ResearchDATING DRY BURN INJURY IN HUMAN PATIENTS BY FLOW CYTOMETRY OF CD4+ AND CD8+ T-CELLS IN THE BLOOD
2021, The Egyptian Journal of Forensic Sciences and Applied Toxicology
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Supported in part by National Institutes of Health grants AI20288, AI41580 and the North Carolina Jaycee Burn Center.
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Reprint requests: Bruce A. Cairns, MD, Department of Surgery, CB# 7210, 165 Burnett-Womack Bldg, University of North Carolina School of Medicine, Chapel Hill, NC 27599-7210.