Droplet-based microfluidic technologies are powerful tools for applications requiring high-throughput, for example, in biochemistry or material sciences. Several systems have been proposed for the high-throughput production of monodisperse emulsions by parallelizing multiple droplet makers. However, these systems have two main limitations: (1) they allow the use of only a single disperse phase; (2) they are based on multiple layer microfabrication techniques. We present here a pipette-and-play solution offering the possibility of manipulating simultaneously 10 different disperse phases on a single layer device. This system allows high-throughput emulsion production using aqueous flow rates of up to 26 ml/h (>110 000 drops/s) leading to emulsions with user-defined complex chemical composition. We demonstrate the multiplex capabilities of our system by measuring the kinetics of β-galactosidase in droplets using nine different concentrations of a fluorogenic substrate.
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Research Article|
May 05 2015
Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays
Jiseok Lim;
Jiseok Lim
a)
1
Droplets, Membranes and Interfaces
; Max Planck Institute for Dynamics and Self-organization, Am Fassberg 17, 37077 Goettingen, Germany
2School of Mechanical Engineering,
Yeungnam University
, Gyeongsan 712-749, South Korea
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Ouriel Caen;
Ouriel Caen
a)
1
Droplets, Membranes and Interfaces
; Max Planck Institute for Dynamics and Self-organization, Am Fassberg 17, 37077 Goettingen, Germany
3
Université Paris Sorbonne Cité
, INSERM UMR-S1147, Centre Universitaire des Saints-Pères, 45 rue des Saints-Pères, 75270 Paris Cedex 06, France
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Jérémy Vrignon;
Jérémy Vrignon
1
Droplets, Membranes and Interfaces
; Max Planck Institute for Dynamics and Self-organization, Am Fassberg 17, 37077 Goettingen, Germany
4CNRS,
Univ. Bordeaux
, CRPP, UPR 8641, 115 Avenue Schweitzer, F-33600 Pessac, France
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Manfred Konrad;
Manfred Konrad
5
Max Planck Institute for Biophysical Chemistry
, Am Fassberg 11, 37077 Goettingen, Germany
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Valérie Taly
;
Valérie Taly
3
Université Paris Sorbonne Cité
, INSERM UMR-S1147, Centre Universitaire des Saints-Pères, 45 rue des Saints-Pères, 75270 Paris Cedex 06, France
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Jean-Christophe Baret
Jean-Christophe Baret
b)
1
Droplets, Membranes and Interfaces
; Max Planck Institute for Dynamics and Self-organization, Am Fassberg 17, 37077 Goettingen, Germany
4CNRS,
Univ. Bordeaux
, CRPP, UPR 8641, 115 Avenue Schweitzer, F-33600 Pessac, France
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a)
J. Lim and O. Caen contributed equally to this work.
b)
Electronic mail: jean-christophe.baret@u-bordeaux.fr
Biomicrofluidics 9, 034101 (2015)
Article history
Received:
January 26 2015
Accepted:
April 15 2015
Citation
Jiseok Lim, Ouriel Caen, Jérémy Vrignon, Manfred Konrad, Valérie Taly, Jean-Christophe Baret; Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays. Biomicrofluidics 1 May 2015; 9 (3): 034101. https://doi.org/10.1063/1.4919415
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