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Horm Metab Res 2007; 39(4): 237-243
DOI: 10.1055/s-2007-972578
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

The Effects of Retinol on Postprandial Parameters in Men with Different FABP2 Promoter Haplotypes

U. Helwig 1 , 2 [*] , D. Rubin 1 , 2 [*] , J. Kiosz 1 , W. Bitter 1 , S. Schreiber 3 , F. Döring 4 , U. R. Fölsch 2 , J. Schrezenmeir 1
  • 1Federal Research Centre for Nutrition and Food, Location Kiel, Hermann-Weigmann Str. 1, Kiel, Germany
  • 2First Department of Medicine, University Hospital Schleswig-Holstein, Schittenhelmstr. 12, Kiel
  • 3Institute for Clinical Molecular Biology, University Hospital Schleswig-Holstein, Schittenhelmstr. 12, Kiel
  • 4Department of Molecular Nutrition, Christian-Albrechts-University, Kiel
Further Information

Publication History

received 5. 10. 2006

accepted 8. 1. 2007

Publication Date:
19 April 2007 (online)

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Abstract

The fatty acid binding protein 2 (FABP2) mediates the intestinal uptake of fatty acids. We and others have identified six FABP2 promoter polymorphisms which result in two haplotypes, A and B. Reporter-gene assays indicated different activity in FABP2 promoter alleles A and B and different responsiveness to PPAR ligands. In silico analysis revealed different putative binding sites in FABP2 haplotypes for retinoid-dependent transcription factors. Therefore, we assumed that retinol supplementation may effect postprandial fat uptake differently in men with FABP2 promoter haplotype A and B. To test this hypothesis, we administered 5000 I.U. retinol/day for 8 weeks to 19 homozygotes for AA and 21 homozygotes for BB and assessed the alteration of postprandial triglycerides during this intervention. FABP2 genotype groups did not significantly differ in anthropometric and laboratory parameters. The alteration of postprandial triglycerides did not differ significantly between genotypes during intervention. This also held true after adjustment for BMI. Furthermore, in a subgroup which had a combination of promoter and common exon polymorphism, the alteration of the postprandial triglycerides did not differ between genotypes. In conclusion, the postprandial triglyceride metabolism of FABP2 promoter AA and BB did not respond differently to retinol administration even though in silico analysis suggested this.

Key words

gene-nutrient interaction - retinol - fatty acid binding protein - postprandial triglyceride metabolism

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1 U H. and D R. contributed equally to the study

Correspondence

U. Helwig

University Hospital Schleswig-Holstein

Campus Kiel

First Medical Department

Schittenhelmstr. 12

24105 Kiel

Phone: +49/431/597 12 96

Fax: +49/431/597 13 02

Email: u.helwig@mucosa.de

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