Download PDF
Planta Med 1999; 65(8): 744-746
DOI: 10.1055/s-2006-960854
Letters

© Georg Thieme Verlag Stuttgart · New York

Xanthine Oxidase Inhibitors from Brandisia hancei

L. D. Kong1 , 3 , J.-L. Wolfender2 , Christopher H. K. Cheng3 , K. Hostettmann2 , Ren Xiang Tan2
  • 1State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, People's Republic of China
  • 2Institute of Pharmacognosy & Phytochemistry, School of Pharmacy, University of Lausanne, BEP, Lausanne-Dorigny, Switzerland
  • 3Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N. T., Hong Kong
Further Information

Publication History

1999

1999

Publication Date:
04 January 2007 (online)

  PDF Download  Permissions and Reprints

Abstract

Xanthine oxidase is a key enzyme associated with the incidence of hyperuricemia-related disorders. Repeated chromatography of the enzyme inhibitory part of the water extract of the twigs and leaves of Brandisia hancei (Scrophulariaceae) gave a flavone luteolin, an iridoid glycoside mussaenoside, two β-sitosterol glycosides daucosterol and β-sitosterol gentiobioside, and five phenylethanoids arenarioside, brandioside, acteoside, 2′-O-acetylacteoside and isoacteoside. Luteolin and isoacteoside inhibited the xanthine oxidase (XO, EC 1.2.3.2) with the IC50 values at 7.83 and 45.48 µM, respectively. Isoacteoside was found to be the first phenylethanoid that decreased substantially the formation of uric acid by inhibiting competitively xanthine oxidase (Ki value: 10.08 µM). Furthermore, the study suggested that the caffeoylation of the 6′-hydroxyl group of the phenylethanoids was essential for the enzyme inhibitory action.

      >