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Horm Metab Res 2003; 35(10): 593-597
DOI: 10.1055/s-2003-43505
Original Clinical
© Georg Thieme Verlag Stuttgart · New York

Differential Effect of Long-term Food Restriction on Fatty Acid Synthase and Leptin Gene Expression in Rat White Adipose Tissue

J.  Korczynska1 , E.  Stelmanska1 , J.  Swierczynski1
  • 1Department of Biochemistry, Medical University of Gdansk, Poland
Further Information

Publication History

Received 13 November 2002

Accepted after second revision 8 July 2003

Publication Date:
07 November 2003 (online)

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Abstract

Long-term food restriction (85 %, 70 % and 50 % of ad libitum energy intake for one month) induced a substantial fall in serum leptin concentration and leptin mRNA levels in epididymal white adipose tissue in rats. Surprisingly, this suppression was not reversed by refeeding ad libitum for 48 h. The reduction in serum leptin concentration and leptin mRNA level did not strictly correlate with reduction in fat or body mass. Unlike serum leptin concentration and epididymal adipose tissue leptin mRNA levels, fatty acid synthase activity, fatty acid synthase protein abundance and fatty acid synthase mRNA levels increased significantly in white adipose tissue after refeeding rats subjected to food restriction. The increase in serum insulin concentration was observed in all groups on different degrees of food restriction and refed ad libitum for 48 h compared to controls. A decrease in serum insulin concentration was found in the rats not refed before sacrifice. Long-term food restriction did not significantly affect serum glucose concentrations in either refed or non-refed rats. The data reported in this paper indicate that there is no rapid rebound in serum leptin concentration or leptin gene expression in contrast to the increase in serum insulin concentration and fatty acid gene expression in white adipose tissue of rats refed ad libitum after one month’s food restriction.

Key words

Leptin - Neuropeptide - Ob gene - Fatty acid synthase - Food restriction - White adipose tissue (rat)

References

  • 1 Penicaud L, Ferre P, Assmacopoulos-Jeannet F, Perderau D, Leturque A, Jeanrenaud B, Picon L, Girard J. Increased gene expression of lipogenic enzyme and glucose tratnsporte in white adipose tissue of suckling and weaned obese Zucker rats.  Biochem J. 1991;  279 303-308
    PubMedGoogle Scholar
  • 2 Guichard C, Dugail I, Le Liepvre X, Lavau M. Genetic regulation of fatty acid synthase expression in adipose tissue:overtranscription of the gene in genetically obese rats.  J Lipid Res. 1992;  33 679-687
    PubMedGoogle Scholar
  • 3 Phillips M S, Liu Q Y, Hammond H A, Dugan V, Hey P J, Caskey C T, Hess J F. Leptin receptor missence mutation in the fatty Zucker rats.  Nat Genet. 1996;  13 18-19
    CrossrefPubMedGoogle Scholar
  • 4 Lee Y, Hirose H, Zhou Y T, Esser V, McGary J D, Unger R H. Increased lipogenic capacity of the islets of obese rats: a role in the pathogenesis of NIDDM.  Diabetes. 1997;  46 408-413
    PubMedGoogle Scholar
  • 5 Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman J. Positional cloning of the mouse obese gene and its human homologue.  Nature. 1994;  372 425-432
    CrossrefPubMedGoogle Scholar
  • 6 Trayhurn P, Duncan J S, Wood A M, Beattie J H. Regulation of metallothionein gene expression and secretion in rats adipocytes differentiated from preadipocytes in primary culture.  Horm Metab Res. 2000;  32 542-547
    PubMedGoogle Scholar
  • 7 Meler C A. Advances in the understanding of the molecular basis of obesity.  Eur J Endocrinol. 1995;  133 761-763
    PubMedGoogle Scholar
  • 8 Campfield L A, Smoth F J, Guisez Y, Devos R, Burn P. Recombinant mouse ob protein: evidence for a peripheral signal linking adiposity and central neural networks.  Science. 1995;  269 546-549
    CrossrefPubMedGoogle Scholar
  • 9 Halaas J L, Gaiwala K S, Maffei M, Cohen S L, Chait B T, Rabinowitz D, Lallone R L, Burley S K, Friedman J M. Weight-reducing effects of the plasma protein encoded by the obese gene.  Science. 1995;  269 543-546
    CrossrefPubMedGoogle Scholar
  • 10 Pelleymounter M, Cullen M J, Baker M B, Hecht R, Winters D, Boone T, Collins F. Effects of the obese gene product on body weight regulation in ob/ob mice.  Science. 1995;  269 540-543
    CrossrefPubMedGoogle Scholar
  • 11 Ahima R S, Flier J S. Leptin.  Annu Rev Physiol. 2000;  62 413-437
    CrossrefPubMedGoogle Scholar
  • 12 Trayhurn P, Thomas M E, Duncan J S, Rayner D V. Effects of fasting and refeeding on ob gene expression in white adipose tissue of lean and obese (ob/ob) mice.  FEBS-Letters. 1995;  368 488-499
    CrossrefPubMedGoogle Scholar
  • 13 Becker D J, Ongemba L N, Brichard V, Henquin J-C, Brichard S M. Diet- and diabetes-induced changes of ob gene expression in rat adipose tissue.  FEBS-Letters. 1995;  371 324-328
    PubMedGoogle Scholar
  • 14 Kochan Z, Karbowska J, Swierczynski J. Unusual increase of lipogenesis in rat white adipose tissue after multiple cycles of starvation-refeeding.  Metabolism. 1997;  46 10-17
    PubMedGoogle Scholar
  • 15 Mizuno T, Bergen H, Kleopoulos S, Bauman W A, Mobbs C V. Effect of nutritional status and aging on leptin gene expression in mice: Importance of glucose.  Horm Metab Res. 1996;  28 679-684
    Article in Thieme ConnectPubMedGoogle Scholar
  • 16 Li H, Matheny M, Nicolson M, Tumer N, Scarpace P J. Leptin gene expression increases with age independent of increasing adiposity in rats.  Diabetes. 1997;  46 2035-2039
    CrossrefPubMedGoogle Scholar
  • 17 Taylor B A, Philips S J. Detection of obesity QTLs on mouse chromosomes 1 and 7 by selective DNA pooling.  Genomics. 1996;  34 389-398
    CrossrefPubMedGoogle Scholar
  • 18 Ma Z, Gingerich R L, Santiago J V, Klein S, Smith C H, Landt M. Radioimmunoassy of leptin in human plasma.  Clin Chem. 1996;  42 942-946
    PubMedGoogle Scholar
  • 19 Karbowska J, Kochan Z, Zelewski L, Swierczynski J. Tissue specific effect of clofibrate on rat lipogenic enzyme gene expression.  Eur J Pharmacol. 1999;  370 329-336
    CrossrefPubMedGoogle Scholar
  • 20 Trayhurn P, Duncan J S, Rayner D V. Acute cold-induced suppression of ob (obese) gene expression in white adipose tissue of mice: mediation by the sympathetic system.  Biochem J. 1995;  311 729-733
    CrossrefPubMedGoogle Scholar
  • 21 Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.  Anal Biochem. 1987;  162 156-159
    PubMedGoogle Scholar
  • 22 Kochan Z, Karbowska J, Swierczynski J. Effect of clofibrate on malic enzyme and leptin mRNA levels in rat brown and white adipose tissue.  Horm Metab Res. 1999;  31 538-542
    PubMedGoogle Scholar
  • 23 Swierczynski J, Korczynska J, Szolkiewicz M, Karbowska J, Kochan Z, Nieweglowski T, Kusiak E, Rutkowski B. Low leptin mRNA level in adipose tissue and normoleptinemia in experimental chronic renal failure.  Exp Nephrol. 2001;  9 54-59
    CrossrefPubMedGoogle Scholar
  • 24 Zelewski M, Swierczynski J. Comparative studies on lipogenic enzyme activities in brown adipose tissue and liver of rat during starvation-refeeding transition and cold exposure.  Comp Biochem Physiol. 1990;  97B 59-63
    PubMedGoogle Scholar
  • 25 Maffei M, Halaas J, Ravussin E, Pratley R E, Lee G H, Zhang Y, Fei H, Kim S, Lallone R, Ranganathan S. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subject.  Nature Med. 1995;  1 1155-1161
    CrossrefPubMedGoogle Scholar
  • 26 Reul B A, Ongemba L N, Pottier A M, Henquin J C, Brichard S M. Insulin and insulin-like growth factor 1 antagonize the stimulation of ob gene expression by dexamethasone in cultured rat adipose tissue. Biochem.  J. 1997;  324 n
    PubMedGoogle Scholar
  • 27 Fukuda H, Iritani N, Sugimoto T, Ikeda H. Transcriptional regulation of fatty acid syntahse gene by insulin/glucose, polyunsaturated fatty acids and leptin in hepatocytes and adipocytes in normal and genetically obese rats.  Eur J Biochem. 1999;  260 505-511
    CrossrefPubMedGoogle Scholar
  • 28 Iritani N, Sugimoto T, Fukuda H. Gene expression of leptin, insulin receptors and lipogenic enzymes are co-ordinately regulated by insulin and dietary fat in rats.  J Nutr. 2000;  130 1183-1188
    PubMedGoogle Scholar
  • 29 Muller G, Ertl J, Gerl M, Preibisch G. Leptin impairs metabolic action of insulin in isolated adipocytes. J.  Biol Chem. 1997;  272 10 585-10 593
    PubMedGoogle Scholar
  • 30 Walder K, Filippis A, Clark S, Zimmet P, Collier G R. Leptin inhibits insulin binding in isolated rat adipocytes.  J Endocrinol. 1997;  155 R5-R7
    CrossrefPubMedGoogle Scholar
  • 31 Bai Y, Zhang S, Kim K-S, Lee J, Kim K-H. Obese gene expression alters the ability of 30A5 preadipocytes to respond to lipogenic hormones.  J Biol Chem. 1996;  271 13 939-13 942
    PubMedGoogle Scholar

J. Swierczynski

Department of Biochemistry, Medical University of Gdansk

ul. Debinki 1 · 80-211 Gdansk, Poland ·

Fax: + 48 (58) 349 1465

Email: juls@amedec.amg.gda.pl

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