Thrombelastographic Effects of Dextran 70 on Human Blood

 

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The effects of dextran was studied by thrombelastography (TEG) because it can graphically depict fibrin, formation and dissolution and plate 1st-fibrin interaction during clothing. Dextran 70 in concentrations of 1-8% ware added to samples of, native whole blood (WB), recalcified whole blood (RWB), platelet-rich plasma (PRP) and platelet-poor plasma (PPP) using buffered saline as control. Dextran produced an 13% reduction in clot Stiffness (MA) with native and celite activated WB. The same native WB shewed a 23? prolongation of clotting (R) and a 24% decrease in the rate of clot formation (α), while the celite activated WB R and α were reduced by 17% and 19% respectively. However, using RWB we found only a 10% reduction in MA as a result of dextran addition, suggesting that citrating and recalcification diminished the dextran effects. With FRF (platelets >560°000 dextran did not show any significant reduction in the measured parameters, uhiU FFP gavs the greatest response with a 36% decrease in MA. Results vere similar if FRF was obtained from subjects who had taken aspirin. This indicates the main action of dextran but to be on the fibrin network rather than on platelets or the plate let-fibrin interaction. though this effect is best seen when platelet concent: rations are low. Clot lysis by urokinase or streptokinase was accelerated in the presence of dextran but was reduced by increased platelet concentrations. These results suggest a direct effect of dextran on fibrin formation and may explain the antithrombotic effect of dextran in thit defective fibrin formation occurs.