Planta Med 2012; 78 - PF90
DOI: 10.1055/s-0032-1320637

Differentiation of Flos Chrysanthemi cultivars by NMR fingerprinting and chemometric analysis

J Zhao 1, M Wang 1, F Wei 4, R Lin 4, TJ Smillie 1, IA Khan 1, 2, 3
  • 1National Center for Natural Products Research
  • 2Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University, MS 38677, USA
  • 3Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
  • 4Research and Inspection Center of Traditional Chinese Medicine and Ethnomedicine, National Institute for the Control of Pharmaceutical & Biological Products, State Food and Drug Administration, 2 Tiantan Xili, 100050 Beijing, China

Flos Chrysanthemi (Ju Hua), the flowerhead of the daisy plant Chrysanthemum morifolium Ramat., has been used as an herbal medicine in TCM for centuries. Recent studies showed that Ju Hua possesses antibacterial, antifungal, antispirochetal, anti-inflammatory, and anti-HIV activities. C. morifolium is native to China. Varieties of C. morifolium plants have been cultivated in China with a long history. In the Chinese Pharmacopeia, four kinds of Ju Hua cultivars, namely “Bo Ju”, “Gong Ju”, “Hang Ju”, “Chu Ju”, are officially recorded under the same title as Ju Hua. Due to the differences in their growing locations, varieties and processing methods, it is believed that the qualities and pharmacologic properties of the different Ju Hua groups may vary, thus their values differ. NMR-based Metabolomics is a potential technology to provide better insight into the qualitative and quantitative shifts in metabolite patterns of the organism. In the present study, we reported the investigation for the differentiation of Flos Chrysanthemi cultivars by using NMR fingerprinting and chemometric analysis.