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DOI: 10.1055/s-0031-1279559
Quantification of the 3-Dimensional Glomeruli Capillary System in Adult Pigs using Micro- and Nano-CT Imaging
Ziele: To evaluate the feasibility for analysis of the kidney glomeruli capillary unit in adult pics using micro- and nano-CT imaging. Methode: Kidneys were harvested from five healthy hybrid pics (6 mo of age; weight 120kg) at a local slaughterhouse and infused with a mixture of BaSO4 and gelatine. Kidney samples (6-mm diameter, 10mm height, n=20) were punched out, harvested and scanned with micro-CT (12µm cubic voxels) and nano-CT (450nm cubic voxels). Diameter and cross-sectional area (µm2) of glomeruli were measured using micro-CT, nano-CT and histology. Moreover, the capillary vessel volume (mm3) within single glomeruli was quantitated using nano-CT. Ergebnis: The glomeruli diameter and cross-sectional area increased significantly as measured by histology, nano-CT and micro-CT (231±30µm, 0.03392 ±0.0091µm2; 252±28µm, 0.04148±0.009µm2; 269±37µm, 0.04763 ±0.01µm2, p<0.001; respectively). Quantitative nano-CT analysis demonstrated a strong linear relationship of the volume of glomeruli and the capillary volume (r2=0.97). Schlussfolgerung: These results demonstrate potential use of quantitative 3D micro- and nano-CT imaging to obtain information about glomeruli dimensions' and vascularisation, which could be useful in animal models of kidney alterations.
Keywords: Imaging, Kidney, Micro-CT, Nano-CT
Korrespondierender Autor: Langheinrich A
Universitätsklinikum Giessen, Radiologie, Klinikstrasse 36, 35385 Giessen
E-Mail: alexander.langheinrich@radiol.med.uni-giessen.de