Planta Med 2010; 76 - P141
DOI: 10.1055/s-0030-1264439

Bioactivity profile of compounds isolated from Hymenocardia acida Tul. leaves

M Sofidiya 1, C Lange 2, I Sattler 2, D Beukes 3, A Afolayan 4, O Odukoya 1, O Familoni 5
  • 1University of Lagos, Department of Pharmacognosy, Faculty of Pharmacy, College of Medicine Campus, Idi-araba, 234 Lagos, Nigeria
  • 2Leibniz Institute for Natural Product Research and Infection Biology-Hans-Knöll-Institute, Department of Biomolecular Chemistry, Department of Biomolecular Chemistry, Beutenbergstrasse 11a, 07745 Jena, Germany
  • 3Rhodes University, Department of Pharmaceutical chemistry, Department of Pharmaceutical chemistry, Rhodes University, 6140 Grahamstown, South Africa
  • 4University of Fort Hare, Department of Botany, Department of Botany, University of Fort Hare, 5700 Alice, South Africa
  • 5University of Lagos, Department of Chemistry, Department of Chemistry, Faculty of Science, University of Lagos, 234 Lagos, Nigeria

In the course of phytochemical investigations to determine the different constituents of H. acida we isolated di (2-ethylhexyl) phthalate (DEPH) and homoorientin. These compounds were subjected to several assays for bioactivity profiling viz: antioxidant, in vitro anti-inflammatory, cytotoxicity and antimicrobial studies. Homoorientin inhibited hydroxyl radicals generation in horse radish assay but failed to inhibit superoxide in xanthine-xanthine assay, while DEPH did not show any antioxidant property. DEPH and Homoorientin showed moderate inhibition of 3α-hydroxysteroid dehydrogenase with an IC50 of 10 and 16µg/ml respectively. The compounds did not show good activity against the micro-organisms tested. The results of this study lend credence to the ethnomedicinal use of H. acida as an anti-inflammatory agent (1) and expand knowledge on the biological activity of its secondary metabolites.

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