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DOI: 10.1055/s-0029-1246787
Effect of anakinra and erythropoietin on infarct size in an ischemia-reperfused rat heart model – first results
Objective: The value of IL-1 receptor antagonists for myocardial preservation has been postulated before. We aimed to evaluate the effect of IL-1 receptor antagonist (anakinra) administration on the infarct size in an ischemia/reperfusion rat heart model and to compare the results with the effects of erythropoietin and a control substance.
Methods: Three groups of rats (n=14) were subjected to 60 minutes of myocardial ischemia followed by 90 minutes of reperfusion and one group of rats (n=4) underwent 60 minutes of ischemia without reperfusion. Anakinra, erythropoietin or the control solution were administered directly prior to the reperfusion period. Myocardial infarct size was determined by triphenyltetrazoliumchlorid staining.
Results: The proportion of the area at risk was comparable in all groups, ranging between 38 and 43.9%.
The infarct size as percentage of the area at risk was 66.8% (±26.6%) in the control group, 52.9% (±15.3%) in the anakinra and 73% (±32.1%) in the erythropoietin group. The infarct extension in the group of animals without reperfusion was 26.8% (±8.7%) of the area at risk.
Conclusion: Our first results point to a decrease in infarct size in rat hearts after administration of anakinra prior to a reperfusion period, whereas erythropoietin seems to increase the infarct size.