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Planta Med 2010; 76(14): 1519-1524
DOI: 10.1055/s-0029-1240991
Pharmacology
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Anti-adipogenic Activities of Alnus incana and Populus balsamifera Bark Extracts, Part II: Bioassay-guided Identification of Actives Salicortin and Oregonin

Louis C. Martineau1 , 3 , Asim Muhammad2 , 3 , Ammar Saleem2 , 3 , Jessica Hervé1 , 3 , Cory S. Harris2 , 3 , John T. Arnason2 , 3 , Pierre S. Haddad1 , 3
  • 1Natural Health Products and Metabolic Diseases Laboratory, Department of Pharmacology, Université de Montréal, Montreal, Quebec, Canada
  • 2Phytochemistry, Medicinal Plant and Ethnopharmacology Laboratory, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada
  • 3Canadian Institutes of Health Research Team in Aboriginal Antidiabetic Medicines, Montreal, Quebec, Canada
Further Information

Publication History

received Sept. 3, 3009 revised January 27, 2010

accepted January 30, 2010

Publication Date:
18 March 2010 (online)

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Abstract

Among modern day metabolic diseases, obesity has reached epidemic proportions worldwide and novel therapeutic support strategies are urgently needed. Adipocytes are interesting targets in this context. Using ethnobotanical and bioassay screening techniques, we have identified two Boreal Forest plants used by the James Bay Cree that potently inhibit adipogenesis, namely Alnus incana ssp. rugosa (Speckled Alder) and Populus balsamifera (Balsam Poplar). The mode of action of this inhibitory activity was reported in a companion paper. The current study report the results of a classical bioassay-guided fractionation approach aimed at identifying the active principles responsible for the inhibition of adipogenesis, as measured using triglyceride accumulation in the 3T3-L1 adipocyte model cell line. The glycosides oregonin and salicortin were isolated and identified as the respective active principles for Alnus incana and Populus balsamifera. These compounds thus offer promise as novel agents to mitigate the incidence or the progression of obesity.

Key words

Alnus incana ssp. rugosa - (Du roi) R. T. Clausen - Betulaceae - Populus balsamifera L. - Salicaceae - aboriginal traditional medicine - 3T3‐L1 adipocyte - salicylate - diarylheptanoid

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Dr. Pierre S. Haddad

Department of Pharmacology
Université de Montréal

P. O. Box 6128 Centre-Ville

Montreal, Quebec H3C 3J7

Canada

Phone: + 1 51 43 43 65 90

Fax: + 1 51 43 43 22 91

Email: pierre.haddad@umontreal.ca

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