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Planta Med 2010; 76(8): 766-772
DOI: 10.1055/s-0029-1240701
Pharmacology
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Comparison of the Enteric Mucosal Immunomodulatory Activity of Combinations of Coptis chinensis Franch. Rhizomes and Evodia rutaecarpa (Juss.) Benth. Fruits in Mice with Dextran Sulphate Sodium-Induced Ulcerative Colitis

Yingying Luo1 , Hongyan Zhao2 , Zhenli Liu2 , Dahong Ju2 , Xiaojuan He3 , Cheng Xiao4 , Guoyue Zhong5 , Shilin Chen6 , Dajian Yang6 , Albert Sun-chi Chan6 , Aiping Lu3 , 6 , 7
  • 1Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, P. R. China
  • 2Institute of Basic Theory of TCM, China Academy of Chinese Medical Sciences, Beijing, P. R. China
  • 3Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing, P. R. China
  • 4Institute of Clinical Medicine, China-Japan Hospital, Beijing, P. R. China
  • 5Chongqing Academy of Chinese Medical Sciences, Chongqing, P. R. China
  • 6State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, Guangdong, P. R. China
  • 7E-Institute of Shanghai Municipal Education Commission, Shanghai TCM University, Shanghai, P. R. China
Further Information

Publication History

received May 10, 2009 revised Nov. 19, 2009

accepted Nov. 23, 2009

Publication Date:
23 December 2009 (online)

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Abstract

Combinations of crude rhizomes of Coptis chinensis Franch., Ranunculaceae, and fruits of Evodia rutaecarpa (Juss.) Benth., Rutaceae, at a ratio of 6 : 1 (formula A) and 1 : 6 (formula B) were extracted with boiling water, and their modulatory activity on enteric mucosal immune responses in mice with dextran sulphate sodium-induced ulcerative colitis was investigated. The results showed that both formulas could reduce the severity of inflammation in the colon. Formula A at a low dose can decrease myeloperoxidase (MPO) activity, and formula B was inactive. Both formulas did not affect the percentages of CD4+ and CD8+ T cells in the periphery, but they evoked an increase of CD8+ T cells among the enteric intraepithelial lymphocytes. Formula B at a low dose could increase both CD4+ and CD8+ cells, and formula A at a high dose could only increase CD8+ T cells among the Peyer's patch lymphocytes (p < 0.05). Both formulas did not affect the percentages of CD4+ and CD8+ T cells among the lamina propria lymphocytes, but decreased the serum concentration of IL-1β (p < 0.05 at a low dose of formula A) and enhanced the level of IL-10 in serum (p < 0.05 at a low dose formula B). We conclude that both formulas have a similar modulating effect on enteric mucosal immune responses, the major difference being that formula A could decrease the level of IL-1 β, while formula B could increase the IL-10 dose in serum.

Key words

Ranunculaceae - Coptis chinensis Franch. - Rutaceae - Evodia rutaecarpa (Juss.) Benth. - ulcerative colitis - mice

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Prof. PhD Aiping Lu MD

Institute of Basic Research in Clinical Medicine
China Academy of Chinese Medical Sciences

100700 Beijing

China

Phone: + 86 10 64 06 76 11

Fax: + 86 10 84 03 28 81

Email: lap64067611@126.com

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