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DOI: 10.1055/s-0029-1210859
© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York
The Effect of Cyclosporin A on Rat Pancreatic B-Cell: Interaction with the Calcium-Antagonist Diltiazem
Publication History
1988
Publication Date:
16 July 2009 (online)
Summary
Cyclosporin A (CyA) was fed to male Wistar rats in a dose of 5, 10 or 50 mg/kg b.wt. for 7 days. In another experimental procedure 10 mg/kg b.wt. CyA was given for 7 days combined with Diltiazem (DIL) 6.25 mg/kg b.wt. once daily ip. Glucose stimulated insulin secretion from the isolated perfused pancreas was investigated in these animals.
Total insulin secretion (ng/50 min) was not affected after feeding 5 mg/kg b.wt. CyA, but was significantly decreased with 10 and 50 mg/kg b.wt. CyA. The biphasic insulin secretion was reduced after 5 mg/kg b.wt. CyA during the initial peak (0—10 min) but not during the second peak (10— 50 min), whereas after 10 and 50 mg/kg b.wt. CyA both peaks nearly reached base-line levels.
DIL markedly increased the initial peak (0—10 min) after feeding CyA 10 mg/kg b.wt., whereas the second peak was not affected.
Our results demonstrate a toxic effect of CyA on the pancreatic B-cell, which might be partly abolished by calcium-antagonists.