ACE-inhibitor Suppresses the Apoptosis Induced by Endoplasmic Reticulum Stress in Renal Tubular in Experimental Diabetic Rats

 

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Abstract

Aims: The aim of the present study was to investigate the role of the endoplasmic reticulum stress and apoptosis in experimental diabetic nephropathy. The effects of ACE inhibitor on the endoplasmic reticulum stress and apoptosis were also assessed.

Methods: Diabetes was induced in male Sprague-Dawley rats by injection with streptozotocin at 60 mg/kg i .p. Diabetic rats were then randomly assigned into control (untreated) or treatment of an ACE inhibitor, perindopril, for 24 weeks. Tubulointerstitial injury was assessed by histopathology. Tubule apoptosis was detected by TUNEL assay. Endoplasmic reticulum stress associated proteins expression of glucose-regulated protein-78 (GRP78/BiP), phospho-eukaryotic initiation factor 2 α (eIF2 α), phospho-pancrenatic ER kinase (PERK) and caspase-12 was assessed by immunohistochemistry and Western blots.

Results: There were more TUNEL-positive nuclei in diabetic kidneys than in control kidneys. At 24 weeks, experimental diabetes was associated with a considerable increase in protein expression of GRP78, phospho-eIF2 α, phospho-PERK, and caspase-12 in the tubulointestitium. ACE inhibitor not only attenuated the apoptosis but also reduced the overexpression of these endoplasmic reticulum stress associated proteins in tubulointestitium of diabetic rats.

Conclusions: Increased tubular apoptosis in experimental diabetic rats is attenuated by blockade of the renin-angiotention system with an ACE inhibitor, which might be in an association with reduced endoplasmic reticulum stress.

References

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1 H. Sun and L. Sun contributed equally to this work.

Correspondence

S. M. Li

Department of Nephrology

Shenzhen Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine

1 Fuhua Road

Futian District

Shenzhen 518033

Guangdong

China

Tel: 86/755/8300 20 10

Fax: 86/755/8835 93 33

Email: shunminli@126.com