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DOI: 10.1055/s-2003-39707
Variation of Podophyllotoxin in Leaves of Eastern Red Cedar (Juniperus virginiana)
Publication History
Received: September 16, 2002
Accepted: January 5, 2003
Publication Date:
12 June 2003 (online)
Abstract
Leaves of Eastern red cedar (Juniperus virginiana L. Cupressaceae) have been reported to contain podophyllotoxin, a pharmaceutical compound used to manufacture drugs for treatment of cancer, rheumatoid arthritis, genital warts, psoriasis, and multiple sclerosis. Podophyllotoxin content of leaves of immature, mature male, and mature female plants (∼1.45 mg·g-1) was significantly higher than that of leaves of juvenile plants (0.60 mg·g-1). Sampling date also affected podophyllotoxin content. Leaves harvested in January and April exhibited higher podophyllotoxin contents (1.56 and 1.45 mg·g-1, respectively) than leaves harvested in February and June (1.06 and 1.08 mg·g-1, respectively). There was no obvious pattern or trend in the data due to sampling date. There was no significant interaction between plant type and sampling date. These results indicate that foliage of mature Eastern red cedar, a waste product of the lumber industry, could be a low-yielding, but relatively stable, source of podophyllotoxin.
References
- 1 Kupchan S M, Hemingway J C, Knox J R. Tumor inhibitors VII. Podophyllotoxin, the active principle of Juniperus virginiana . J Pharm Sci. 1965; 54 659-60
-
2 Bedir E, Khan I, Moraes R M. Bioprospecting for podophyllotoxin. In: Janick J, Whipkey A, editors
Trends in New Crops and New Uses . ASHS Press Alexandria, Va; 2002: pp. 546-50 - 3 Stähelin H F, von Wartburg A. The chemical and biological route from podophyllotoxin glucoside to etoposide: Ninth Cain Memorial Lecture. Cancer Res. 1991; 51 5-15
- 4 Giri A, Narasu M L. Production of podophyllotoxin from Podophyllum hexandrum: A potential natural product for clinically useful anticancer drugs. Cytotechnology. 2000; 34 17-26
- 5 Lerndal T, Svensson B. A clinical study of CPH 82 vs methotrexate in early rheumatoid arthritis. Rheumatology (Oxford). 2000; 39 316-20
-
6 Beutner K R. Podophyllotoxin in the treatment of genital warts. In: Elsner P, Eichmann A, editors
Sexually Transmitted Diseases. Advances in Diagnosis and Treatment . Curr Probl Dermatol Vol. 24. Basel; Karger 1996: pp. 227-32 - 7 Foster S. Medicinal plant conservation and genetic resources: examples from the temperate Northern hemisphere. Acta Hort. 1993; 330 67-73
- 8 Canel C, Dayan F E, Ganzera M, Khan I A, Rimando A, Burandt J r. CL, et al. High yield of podophyllotoxin from leaves of Podophyllum peltatum by in situ conversion of podophyllotoxin 4-O-β-D-glucopyranoside. Planta Med. 2001; 67 97-9
- 9 Bastos J K, Burandt CL J r, Nanayakkara N PD, Bryanat L, McChesney J D. Quantitation of aryltetralin lignans in plant parts and among different populations of Podophyllum peltatum by reverse-phase high-performance liquid chromatography. J Nat Prod. 1996; 59 406-8
Dr. Kent E. Cushman
North Mississippi Research & Extension Center
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MS 38879
USA
Email: kcushman@ra.msstate.edu
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