Original ResearchFull Report: Basic and Translational—Alimentary TractIn Vitro Expansion of Human Gastric Epithelial Stem Cells and Their Responses to Bacterial Infection
Full Report: Basic and Translational—Alimentary Tract
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Keywords
Stomach Cancer
Gastric Epithelium
Primary Cells
Tissue Engineering
Abbreviations used in this paper
cagPAI
cytotoxicity-associated gene pathogenicity island
CDX
caudal-type homeobox
DMEM
Dulbecco’s modified Eagle medium
EGF
epidermal growth factor
ENRWFG
EGF, noggin, R-spondin1, Wnt, FGF10, and gastrin
FGF
fibroblast growth factor
GSK3β
glykogensynthase-Kinase 3 beta
IGF
insulin-like growth factor
LPS
lipopolysaccharide
MOI
multiplicity of infection
MUC
mucin
ODN
oligodeoxynucleotide
PCR
polymerase chain reaction
PGC
pepsinogen
PGE
prostaglandin E
SST
somatostatin
TFF
trefoil factor
TGF
transforming growth factor
TNF
tumor necrosis factor
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Conflicts of interest These authors disclose the following: Hans Clevers and Meritxell Huch hold patents for organoid culture. The remaining authors disclose no conflicts.
Funding Supported by an EU Marie Curie Fellowship (EU/300686-InfO to S.B. and EU/236954-ICSC-Lgr5 to M.H.), an EU FP7 grant EU/232814-StemCellMark (M.v.d.W. and R.V.), a National Institutes of Health/Massachusetts Institute of Technology subaward 5710002735 (H.B.), and a Research Prize from the United European Gastroenterology Foundation (H.C.).
Author names in bold designate shared co-first authors.