Basic-alimentary tractRectal Instillation of Butyrate Provides a Novel Clinically Relevant Model of Noninflammatory Colonic Hypersensitivity in Rats
Section snippets
Animals
Male and female Sprague-Dawley rats (Charles River, L’Arbresle, France) weighing 200–220 g were used in this study. Rats were maintained in laboratory conditions for 1 week before each experiment. The animals were housed 5 per cage with food and water available ad libitum. All studies were performed in accordance with the proposal of the Committee for Research and Ethical Issues of the International Association for the Study of Pain.22 Great care was taken, particularly with regard to housing
Effect of butyrate enemas on colonic sensitivity and on lumbar von Frey scores
Butyrate induced a significant concentration-dependent decrease of thresholds to CRD (Figure 2A) and of von Frey scores (Figure 2C) in 100% of butyrate-instilled animals, while scores for saline-treated male rats remained stable throughout the experiment (50.6 ± 2.1 mm Hg and 45.7 ± 1.3 mN for CRD and von Frey scores, respectively). A significant decrease in CRD thresholds was observed for the lowest butyrate concentration (8 mmol/L) at days 3 and 6 after the start of butyrate enemas. The
Discussion
This study clearly shows that colonic butyrate enemas induce a hypersensitivity to CRD characterized (1) by a decrease in pressure that induced a clearly visible abdominal contraction (pain behavioral response), (2) by a shift to the left of the curve of the scores of abdominal pain (method of Al Chaer et al5) induced by increasing distention pressures, and (3) by a referred cutaneous lumbar hyperalgesia assessed using the von Frey test. This result, together with the nature of the inducing
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Supported by an Action Thématique Concertée (ATC) scientific research grant (Nutrition 2002, no. ASE2128CSA).