Original ContributionsThe expression of E-cadherin and catenins in sporadic colorectal carcinoma*,**
Section snippets
Human tissue
Formalin-fixed, paraffin-embedded tissue from 30 patients with sporadic colorectal tumors was obtained from the archived material at the Department of Histopathology, Hammersmith Hospital, London, England. The material included 30 primary adenocarcinomas (13 cases with lymph node deposits). The patients were 20 men whose ages ranged from 54 to 86 years with a median age of 69 years, and 10 women whose ages ranged from 44 to 79 years with a median age of 62 years. The adenocarcinomas were graded
Expression of E-cadherin and catenins in histologically normal colorectal epithelium
Membranous staining for E-cadherin and α-, β-, and γ-catenin was seen at the intercellular borders of the histologically normal colorectal epithelium. There was also weak cytoplasmic staining for the 4 proteins.
Expression of E-cadherin and the catenins in colorectal carcinomas and their metastatic deposits
Table 1 summarizes the immunoexpression of E-cadherin and catenins in the 30 carcinomas compared with the nonneoplastic mucosa.Empty Cell Membrane Cytoplasm Nucleus Protein Normal Decreased Normal Increased Empty Cell
Discussion
Several immunohistochemical studies have compared the expression of E-cadherin8, 16, 19 and catenins27, 28 in colorectal neoplasms with those in the normal adjacent colorectal mucosa. Most show an overall decrease in the expression of E-cadherin, which was inversely proportional to the degree of tumor differentiation. In carcinomas, a reciprocal redistribution of E-cadherin from the plasma membrane to the cytoplasm occurs.19 For the catenins, overall decreased expression has commonly been
Acknowledgements
The authors thank the United Kingdom Human Genome Mapping Project Resource Centre for kindly providing the IMAGE Consortium clones used for riboprobe preparation.
References (47)
- et al.
Identification of a neural alpha-catenin as a key regulator of cadherin function and multicellular organization
Cell
(1992) - et al.
Genetic manipulation of E-cadherin expression by epithelial tumor cells reveals an invasion suppressor role
Cell
(1991) - et al.
Immunohistochemical analysis of E-cadherin expression in human colorectal tumours
Pathol Res Pract
(1993) - et al.
Abnormal immunoreactivity of the E-cadherin-catenin complex in gastric carcinoma: relationship with patient survival
Gastroenterology
(1997) - et al.
Catenins as mediators of the cytoplasmic functions of cadherins
J Cell Sci
(1993) - et al.
Uvomorulin-catenin complex formation is regulated by a specific domain in the cytoplasmic region of the cell adhesion molecule
Proc Natl Acad Sci USA
(1990) - et al.
Loss of E-cadherin-dependent cell-cell adhesion due to mutation of the beta-catenin gene in a human cancer cell line, HSC-39
Mol Cell Biol
(1995) - et al.
E-cadherin-mediated cell-cell adhesion prevents invasiveness of human carcinoma cells
J Cell Biol
(1991) - et al.
Dissecting tumor cell invasion: Epithelial cells acquire invasive properties after the loss of uvomorulin-mediated cell-cell adhesion
J Cell Biol
(1989) - et al.
L-CAM expression in normal, premalignant, and malignant colon mucosa
J Pathol
(1992)
Expression and potential role of E-cadherin in pancreatic carcinoma
Int J Pancreatol
E-cadherin and alpha-catenin expression in human esophageal cancer
Cancer Res
Altered expression of E-cadherin in gastric cancer tissues and carcinomatous fluid
Br J Cancer
Immunohistochemical evaluation of E-cadherin adhesion molecule expression in human gastric cancer
Virchows Arch A Pathol Anat Histopathol
E-cadherin expression in primary and metastatic gastric cancer. Down-regulation correlates with cellular dedifferentiation and glandular disintegration
Cancer Res
The role of the cell-cell adhesion molecule E-cadherin in large bowel tumour cell invasion and metastasis
Br J Cancer
Loss of cell-cell and cell-matrix adhesion molecules in colorectal cancer
Br J Cancer
E-cadherin expression in colorectal cancer. An immunocytochemical and in situ hybridization study
Am J Pathol
L-CAM expression in lymph node and liver metastases of colorectal carcinomas
J Pathol
Changes in E-cadherin immunoreactivity in the adenoma-carcinoma sequence of the large bowel
Virchows Arch
Immunohistochemical detection of alpha-catenin expression in human cancers
Am J Pathol
Immunohistochemical evaluation of alpha-catenin expression in human gastric cancer
Virchows Arch
Beta-catenin expression in human cancers
Am J Pathol
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Supported in part by a Locally Organized Clinical Research Scheme grant from the Hammersmith Hospital NHS Trust and the Imperial Cancer Research Fund. Mona A. El-Bahrawy is supported by a scholarship from the Egyptian government.
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Address correspondence and reprint requests to Malcolm R. Alison, DSc, Histopathology Department, Hammersmith Hospital, London, W12 ONN, England.