Liver, Pancreas, and Biliary TractDifferential gene expression between chronic hepatitis B and C hepatic lesion☆
Section snippets
Patients
The subjects were 12 patients with chronic hepatitis B, 14 patients with chronic hepatitis C, and 6 patients who showed no clinical signs of hepatitis in Kanazawa University Hospital, Japan, between 1997 and 1999 (Table 1).The last 6 patients were seronegative for either hepatitis B surface antigen (HBsAg) or HCV antibody and had liver function values within the normal limits. Tissue samples from these 6 patients were surgically obtained from the noncancerous parts of the liver complicated with
Establishment of a cDNA microarray technique for gene expression profiling in liver biopsy samples
Because the amount of mRNA recovered from small biopsy samples was not sufficient for cDNA microarray analysis, aRNA amplification of samples was necessary. To evaluate the sensitivity and reproducibility of cDNA microarray profiling using aRNA amplification, we performed preliminary experiments on a small scale, with 64 selected genes and mRNA from tissue culture cell lines. We could amplify RNA using 20 ng of mRNA as a template from THLE5-b and Huh-7 cells and used the amplified products for
Discussion
As a breakthrough technology for the development of modern functional research in human genome-based science, cDNA microarray has become one of the most powerful and sensitive techniques that can be broadly applied to both basic and clinical research. The cDNA microarray method makes it practical to quantitatively measure expression levels of hundreds to thousands of genes in parallel and has been used successfully to observe alterations and variations of gene expression in a variety of tissue
Acknowledgements
The authors thank Dr. Chris Barry for practical and productive technical advice on the microarray technique; Dr. Yasunari Nakamoto for discussion concerning the manuscript; Dr. Katsuhisa Inamura, Dr. Kazunori Kawaguchi, Dr. Katsuhiko Higuchi, Akemi Nakano, Junko Kitagawa, and Masami Ueda for excellent technical assistance; and Professor Seishi Murakami, Associate Professor Motoaki Ohtsubo, and Dr. Nobuyuki Itoh for providing cDNA clones.
References (45)
- et al.
Characteristic difference of hepatocellular carcinoma between hepatitis B and C viral infection in Japan
Hepatology
(1995) - et al.
Disease progression and hepatocellular carcinogenesis in patients with chronic viral hepatitis: a prospective observation of 2215 patients
J Hepatol
(1998) - et al.
Cell cycle regulation of hepatitis C virus internal ribosomal entry site-directed translation
Gastroenterology
(2000) - et al.
Cytoplasmic dynein and dynactin in cell division and intracellular transport
Curr Opin Cell Biol
(1999) - et al.
The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex
Mol Cell
(1999) - et al.
The E2F transcription factor is a cellular target for the RB protein
Cell
(1991) - et al.
Different cytokine profiles of intrahepatic T cells in chronic hepatitis B and hepatitis C virus infections
Gastroenterology
(1997) - et al.
A mechanism of repression by acute myeloid leukemia-1, the target of multiple chromosomal translocations in acute leukemia
J Biol Chem
(2000) - et al.
Expression of the AML-1 oncogene shortens the G(1) phase of the cell cycle
J Biol Chem
(2000) - et al.
The hepatitis B virus X protein is a co-activator of activated transcription that modulates the transcription machinery and distal binding activators
J Biol Chem
(1998)
Oncoprotein MDM2 is a ubiquitin ligase E3 for tumor suppressor p53
FEBS Lett
Quantitative monitoring of gene expression patterns with a complementary DNA microarray
Science
Parallel human genome analysis: microarray-based expression monitoring of 1000 genes
Proc Natl Acad Sci U S A
Use of a cDNA microarray to analyse gene expression patterns in human cancer
Nat Genet
Gene expression profiling of alveolar rhabdomyosarcoma with cDNA microarrays
Cancer Res
The transcriptional program in the response of human fibroblasts to serum
Science
Yeast microarrays for genome wide parallel genetic and gene expression analysis
Proc Natl Acad Sci U S A
The transcriptional program of sporulation in budding yeast
Science
Comprehensive identification of cell cycle–regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization
Mol Biol Cell
Exploring drug-induced alterations in gene expression in Mycobacterium tuberculosis by microarray hybridization
Proc Natl Acad Sci U S A
Discovery and analysis of inflammatory disease–related genes using cDNA microarrays
Proc Natl Acad Sci U S A
In vivo gene expression profile analysis of human breast cancer progression
Cancer Res
Cited by (0)
- ☆
Address requests for reprints to: Masao Honda, M.D., Ph.D., First Department of Internal Medicine, School of Medicine, Kanazawa University, Takara-Machi 13-1, Kanazawa, 920-8641, Japan. e-mail: [email protected]; fax: (81) 76-234-4250.