In vitro simulated digestion and fecal fermentation of exopolysaccharides from Lacticaseibacillus paracasei GL1

Xiaomeng Wang; Juanjuan Tian; Nanyu Tang; Xueliang Zhang; Luyao Xiao; Mengjia Xu; Mingsheng Dong; Wei Li

doi:10.1039/D3FO00676J

In vitro simulated digestion and fecal fermentation of exopolysaccharides from Lacticaseibacillus paracasei GL1†

Xiaomeng Wang,^a Juanjuan Tian,^a Nanyu Tang,^a Xueliang Zhang,^a Luyao Xiao,^a Mengjia Xu,^abc Mingsheng Dong^a and Wei Li

*^a

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* Corresponding authors

^a Sanya Institute of Nanjing Agricultural University, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, P.R. China
E-mail: lw1981@njau.edu.cn
Tel: +86 25 84399910

^b Affiliated Cixi Hospital, Wenzhou Medical University, Cixi 315300, Zhejiang, P.R. China

^c Zhejiang International Scientific and Technological Cooperative Base of Biomedical Materials and Technology, Zhejiang Engineering Research Center for Biomedical Materials, Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, P.R. China

Abstract

The prebiotic properties of two purified fractions (GL1-E1 and GL1-E2) of exopolysaccharides (EPSs) from Lacticaseibacillus paracasei GL1 were investigated through in vitro fermentation of pure and human fecal cultures. The results indicated that the simulated digestion under saliva, gastric, and small intestinal conditions had no effect on GL1-E1 and GL1-E2. Additionally, GL1-E1 and GL1-E2 can be used as substrates for Lactobacillus and Lactococcus growth. It was also found that both were gradually degraded and utilized by the gut microbiota. As fermentation proceeded, the pH continued to decrease. Additionally, the total short-chain fatty acid (SCFA) production significantly increased, especially the major SCFA of formic, lactic, and acetic acid. Furthermore, GL1-E1 and GL1-E2 could significantly regulate the composition of the gut microbiota, by increasing the relative abundances of Bacteroides and Phascolarctobacterium, and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella, and Fusobacterium. These results suggest that GL1-E1 and GL1-E2 have the potential to be developed as a prebiotic.

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DOI: https://doi.org/10.1039/D3FO00676J
Article type: Paper
Submitted: 17 Feb 2023
Accepted: 12 Apr 2023
First published: 14 Apr 2023

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Food Funct., 2023,14, 5120-5137

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In vitro simulated digestion and fecal fermentation of exopolysaccharides from Lacticaseibacillus paracasei GL1

X. Wang, J. Tian, N. Tang, X. Zhang, L. Xiao, M. Xu, M. Dong and W. Li, Food Funct., 2023, 14, 5120 DOI: 10.1039/D3FO00676J

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In vitro simulated digestion and fecal fermentation of exopolysaccharides from Lacticaseibacillus paracasei GL1†

Abstract

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In vitro simulated digestion and fecal fermentation of exopolysaccharides from Lacticaseibacillus paracasei GL1

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