Standard-free single magnetic bead evaluation: a stable nanoplatform for prostate disease differentiation

Zili Huang; Xiaobo Xie; Bei Xu; Rui Liu; Jianyu Hu; Yi Lv

doi:10.1039/D2SC00928E

Standard-free single magnetic bead evaluation: a stable nanoplatform for prostate disease differentiation†

Zili Huang,

^a Xiaobo Xie,^b Bei Xu,^c Rui Liu,

^a Jianyu Hu^d and Yi Lv

*^ab

Author affiliations

* Corresponding authors

^a Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, Chengdu, PR China
E-mail: lvy@scu.edu.cn

^b Analytical & Testing Center, Sichuan University, Chengdu, PR China

^c Department of Clinical Laboratory, Mianyang Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Mianyang, PR China

^d Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, Canada

Abstract

Explicit interpretation of heterogeneity between prostate-specific antigen (PSA) subtypes is essential for prostate cancer differentiation during different disease courses, whereas a universal protocol with uniform criteria is still lacking across the globe. In this work, a standard-free single magnetic bead (SMB) nanoplatform utilizing metal nanoparticles with optimal diameters was proposed for prostate disease differentiation in a 134-donor model. The inaccuracy of detection in absolute quantification was diminished via evaluations of metal intensities on the single magnetic bead. The intrinsic proportion of fPSA in tPSA was successfully evaluated by direct use of the Pt to Au intensity ratio (Pt/Au ratio), exhibiting better differentiation between healthy and unhealthy, benign prostatic hyperplasia (BPH) and cancer individuals compared with solo fPSA or tPSA. We generated thresholds respectively for prostate disease differentiation, envisioning that this standard-free SMB nanoplatform would establish a standardized methodology with uniform criteria worldwide in cancer diagnosis, staging, and postoperative assessments.

This article is part of the themed collection: Most popular 2022 analytical chemistry articles

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DOI: https://doi.org/10.1039/D2SC00928E
Article type: Edge Article
Submitted: 14 Feb 2022
Accepted: 28 Apr 2022
First published: 29 Apr 2022
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry

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Chem. Sci., 2022,13, 6270-6275

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Standard-free single magnetic bead evaluation: a stable nanoplatform for prostate disease differentiation

Z. Huang, X. Xie, B. Xu, R. Liu, J. Hu and Y. Lv, Chem. Sci., 2022, 13, 6270 DOI: 10.1039/D2SC00928E

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Chemical Science

Standard-free single magnetic bead evaluation: a stable nanoplatform for prostate disease differentiation†

Abstract

Supplementary files

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Standard-free single magnetic bead evaluation: a stable nanoplatform for prostate disease differentiation

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