Dietary leucine and fish oil cooperatively regulate skeletal myofiber type transformation via the CaMKII signaling pathway of pigs

Saiming Gong; Yunju Yin; Mengmeng Han; Liu Guo; Yehui Duan; Qiuping Guo; Jie Yin; Fengna Li

doi:10.1039/D2FO03338K

Dietary leucine and fish oil cooperatively regulate skeletal myofiber type transformation via the CaMKII signaling pathway of pigs†

Saiming Gong,^ab Yunju Yin,^ab Mengmeng Han,^bc Liu Guo,^bc Yehui Duan,^b Qiuping Guo,^b Jie Yin*^a and Fengna Li

*^bd

Author affiliations

* Corresponding authors

^a College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China
E-mail: yinjie@hunau.edu.cn

^b Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process; Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha 410125, China
E-mail: lifengna@isa.ac.cn

^c University of Chinese Academy of Sciences, Beijing 100049, China

^d College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China

Abstract

The study investigated the effects of dietary leucine (Leu) and fish oil (FO) on skeletal myofiber type transformations in pigs and their potential interactions. The results showed that Leu increased the content of Leu, upregulated myocyte enhancer factor-2C (MEF2C) and activated the CaMKII-AMPK/SIRT1-PGC-1α pathway in the longissimus dorsi (LD) muscle. FO increased adiponectin and fatty acid beta-oxidation of LD muscle. Activation of the adiponectin signaling pathway by FO further enhanced the CaMKII pathway and upregulated the expression of MEF2C. Moreover, we found that Leu increased cyclic AMP and caffeine, and FO increased linoleic acid and glutamine in muscle metabolites, which may be the cause of myofiber conversion. In conclusion, this study demonstrated that dietary Leu and FO co-regulated the transformation from glycolytic to oxidative skeletal myofiber type. It is hypothesized that there is an interaction between amino acids and polyunsaturated fatty acids, possibly via the CaMKII signaling pathway to upregulate MEF2 and mitochondrial biogenesis.

This article is part of the themed collection: Food & Function HOT Articles 2023

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DOI: https://doi.org/10.1039/D2FO03338K
Article type: Paper
Submitted: 04 Nov 2022
Accepted: 05 Dec 2022
First published: 09 Dec 2022

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Food Funct., 2023,14, 133-147

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Dietary leucine and fish oil cooperatively regulate skeletal myofiber type transformation via the CaMKII signaling pathway of pigs

S. Gong, Y. Yin, M. Han, L. Guo, Y. Duan, Q. Guo, J. Yin and F. Li, Food Funct., 2023, 14, 133 DOI: 10.1039/D2FO03338K

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Food & Function

Dietary leucine and fish oil cooperatively regulate skeletal myofiber type transformation via the CaMKII signaling pathway of pigs†

Abstract

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Dietary leucine and fish oil cooperatively regulate skeletal myofiber type transformation via the CaMKII signaling pathway of pigs

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