Issue 4, 2023
From the journal:

Analyst

Supersensitive detection of single-histamine molecule on nanoplates by turn-on small molecule fluorescence sandwich immunoassay†

Seungah Lee,‡a   Junghwa Lee‡b  and  Seong Ho Kang ORCID logo *ab  

* Corresponding authors

a Department of Applied Chemistry and Institute of Natural Sciences, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Republic of Korea
E-mail: shkang@khu.ac.kr

b Department of Chemistry, Graduate School, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Republic of Korea

Abstract

We develop a supersensitive “turn-on format” fluorescence sandwich immunoassay for detecting small single molecules. Gold nanoplate-based biotin antibodies and streptavidin–fluorophores were used instead of streptavidin–horseradish peroxidase reacting with a biotin tracer in a microplate-based competitive enzyme-linked immunosorbent assay (ELISA). Our platform showed a low detection limit of 5 zeptomolar (5 × 10–21 M), 5.4 × 1010 times higher detection sensitivity than the conventional tune-off format ELISA.

Graphical abstract: Supersensitive detection of single-histamine molecule on nanoplates by turn-on small molecule fluorescence sandwich immunoassay

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Article information

DOI
https://doi.org/10.1039/D2AN01734B
Article type
Communication
Submitted
22 Oct 2022
Accepted
20 Jan 2023
First published
20 Jan 2023

Analyst, 2023,148, 714-718

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Supersensitive detection of single-histamine molecule on nanoplates by turn-on small molecule fluorescence sandwich immunoassay

S. Lee, J. Lee and S. H. Kang, Analyst, 2023, 148, 714 DOI: 10.1039/D2AN01734B

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