Issue 12, 2021
From the journal:

Food & Function

The absorption of glycosaminoglycans of different molecular weight obtained from Apostichopus japonicus: an in vitro and in situ study

Mengjie Ma,a   Han Wang,b   Yongkai Yuan,c   Yuanhong Wang,ad   Shuang Yang ORCID logo *ad  and  Zhihua Lv ORCID logo *ad  

* Corresponding authors

a Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao, Shandong Province, People's Republic of China
E-mail: yangshuang@ouc.edu.cn, lvzhihua@ouc.edu.cn
Tel: +86 532 82032064

b Key Laboratory of Chemistry of Northwestern Plant Resources, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, Gansu Province, People's Republic of China

c School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China

d Laboratory of Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology, Qingdao, Shandong Province, People's Republic of China

Abstract

The purpose of this study was to investigate the absorption and transport of glycosaminoglycan from Apostichopus japonicus (AHG) and its depolymerized derivatives (DAHG-1, DAHG-2, DAHG-3). The AHG and depolymerized AHGs (DAHGs) were characterized by high-performance gel permeation chromatography (HPGPC), Raman spectroscopy and atomic force microscopy (AFM). The results showed that there was no significant difference of an AHG primary structure and functional groups during the depolymerization. Meanwhile, AFM observation showed that AHG and DAHGs possessed linear structures. In this study, a rapid and sensitive liquid chromatographic post-column derivatization method was used to investigate the absorption of AHG and DAHGs with the Caco-2 cell model, the M cell model and the intestinal recirculating perfusion. It was found that AHG and DAHGs can be absorbed in the intestine, and their transport involved endocytosis.

Graphical abstract: The absorption of glycosaminoglycans of different molecular weight obtained from Apostichopus japonicus: an in vitro and in situ study

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Article information

DOI
https://doi.org/10.1039/D1FO00624J
Article type
Paper
Submitted
28 Feb 2021
Accepted
02 May 2021
First published
06 May 2021

Food Funct., 2021,12, 5551-5562

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The absorption of glycosaminoglycans of different molecular weight obtained from Apostichopus japonicus: an in vitro and in situ study

M. Ma, H. Wang, Y. Yuan, Y. Wang, S. Yang and Z. Lv, Food Funct., 2021, 12, 5551 DOI: 10.1039/D1FO00624J

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