Synthesis and properties of the anticodon stem-loop of human mitochondrial tRNAMet containing the disease-related G or m1G nucleosides at position 37

Karolina Podskoczyj; Katarzyna Kulik; Joanna Wasko; Barbara Nawrot; Tsutomu Suzuki; Grazyna Leszczynska

doi:10.1039/D1CC05215B

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Synthesis and properties of the anticodon stem-loop of human mitochondrial tRNA^Met containing the disease-related G or m¹G nucleosides at position 37†

Karolina Podskoczyj,

^a Katarzyna Kulik,

^b Joanna Wasko,

^a Barbara Nawrot,

^b Tsutomu Suzuki

^c and Grazyna Leszczynska

*^a

Author affiliations

* Corresponding authors

^a Institute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, Lodz 90-924, Poland
E-mail: grazyna.leszczynska@p.lodz.pl
Tel: +48 426313150

^b Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Division of Bioorganic Chemistry, Sienkiewicza 112, Lodz 90-363, Poland

^c Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan

Abstract

A single point mutation (A4435G) in the human mitochondrial tRNA^Met (hmt-tRNA^Met) gene causes severe mitochondrial disorders associated with hypertension, type 2 diabetes and LHON. This mutation leads to the exchange of A₃₇ in the anticodon loop of hmt-tRNA^Met for G₃₇ and 1-methylguanosine (m¹G₃₇). Here we present the first synthesis and structural/biophysical studies of the anticodon stem and loop of pathogenic hmt-tRNAs^Met.

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Article information

DOI: https://doi.org/10.1039/D1CC05215B
Article type: Communication
Submitted: 15 Sep 2021
Accepted: 28 Oct 2021
First published: 28 Oct 2021

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Chem. Commun., 2021,57, 12540-12543

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Synthesis and properties of the anticodon stem-loop of human mitochondrial tRNA^Met containing the disease-related G or m¹G nucleosides at position 37

K. Podskoczyj, K. Kulik, J. Wasko, B. Nawrot, T. Suzuki and G. Leszczynska, Chem. Commun., 2021, 57, 12540 DOI: 10.1039/D1CC05215B

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Chemical Communications