Effective ACE2 peptide–nanoparticle conjugation and its binding with the SARS-Cov-2 RBD quantified by dynamic light scattering

Vince St. Dollente Mesias; Hongni Zhu; Xiao Tang; Xin Dai; Yusong Guo; Wei Liu; Jinqing Huang

doi:10.1039/D1CC02267A

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Effective ACE2 peptide–nanoparticle conjugation and its binding with the SARS-Cov-2 RBD quantified by dynamic light scattering†

Vince St. Dollente Mesias,

^a Hongni Zhu,^a Xiao Tang,

^b Xin Dai,^a Yusong Guo,^b Wei Liu

^c and Jinqing Huang

*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
E-mail: jqhuang@ust.hk

^b Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China

^c Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China

Abstract

The infection of coronavirus initiates with the binding between its spike protein receptor binding domain (RBD) and a human cellular receptor called angiotensin-converting enzyme 2 (ACE2). Here, we construct truncated ACE2 peptide-conjugated gold nanoparticles as antiviral scaffolds and study their binding with the SARS-CoV-2 RBD using dynamic light scattering (DLS). Systematic DLS analysis identifies the effective peptide–nanoparticle conjugation and its efficient, specific, and long-lasting multivalent binding towards the RBD with a binding affinity of 41 nM, indicating the potential of this antiviral platform to compete with natural ACE2–RBD interactions for viral blocking and showcasing an accessible approach to measure the binding constants and kinetics.

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Article information

DOI: https://doi.org/10.1039/D1CC02267A
Article type: Communication
Submitted: 28 Apr 2021
Accepted: 19 Jun 2021
First published: 21 Jun 2021

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Chem. Commun., 2021,57, 6979-6982

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Effective ACE2 peptide–nanoparticle conjugation and its binding with the SARS-Cov-2 RBD quantified by dynamic light scattering

V. St. D. Mesias, H. Zhu, X. Tang, X. Dai, Y. Guo, W. Liu and J. Huang, Chem. Commun., 2021, 57, 6979 DOI: 10.1039/D1CC02267A

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Chemical Communications

Effective ACE2 peptide–nanoparticle conjugation and its binding with the SARS-Cov-2 RBD quantified by dynamic light scattering†

Abstract

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Effective ACE2 peptide–nanoparticle conjugation and its binding with the SARS-Cov-2 RBD quantified by dynamic light scattering

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