SCHEDULED MAINTENANCE
A ratiometric electrochemical microsensor for monitoring chloride ions in vivo†
Abstract
Chloride ion (Cl−), the most common anion in animal brain, has been verified to play a vital role in maintaining normal physiological processes. Thus, development of a reliable platform to determine Cl− is of great significance for brain research involving Cl−. In this work, a ratiometric electrochemical microsensor (REM) for the in vivo measurement of cerebral Cl− was designed. To prepare REM, uniform Ag nanoparticles (Ag NPs) with nano-level sizes were synthesized via an adsorption–reduction process, which served as selective recognition elements for Cl− determination, while methylene blue (MB) was absorbed and acted as an inner reference unit to avoid the environmental interference of complicated brain systems. As a result, this developed REM exhibited high sensitivity and selectivity, as well as good stability, reproducibility and anti-biofouling. This reliable approach was established to monitor Cl− in mouse brain.
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