Issue 19, 2020

Heterologous expression of the trichostatin gene cluster and functional characterization of N-methyltransferase TsnB8

Abstract

Trichostatins are potent inhibitors of histone deacetylase (HDAC). In this work, a new trichostatin derivative isotrichostatin RK (1) and five known compounds trichostatin RK (2), JBIR-111 (3), 9179B (4), trichostatic acid (5) and trichostatin A (6) were isolated from marine-derived Streptomyces sp. SCSIO 40028. The biosynthetic gene cluster (tsnB) for trichostatins was identified from Streptomyces sp. SCSIO 40028 and validated by heterologous expression in Streptomyces lividans TK64. N-Methyltransferase TsnB8 was demonstrated to catalyze successive methyltransfer reactions by in vivo gene inactivation and in vitro enzyme assays.

Graphical abstract: Heterologous expression of the trichostatin gene cluster and functional characterization of N-methyltransferase TsnB8

Supplementary files

Article information

Article type
Communication
Submitted
24 Mar 2020
Accepted
23 Apr 2020
First published
23 Apr 2020

Org. Biomol. Chem., 2020,18, 3649-3653

Heterologous expression of the trichostatin gene cluster and functional characterization of N-methyltransferase TsnB8

W. Liu, V. G. Jannu, Z. Liu, Q. Zhang, X. Jiang, L. Ma, W. Zhang, C. Zhang and Y. Zhu, Org. Biomol. Chem., 2020, 18, 3649 DOI: 10.1039/D0OB00617C

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