Far-red imaging of β-galactosidase through a phospha-fluorescein

Zichao Ding; Chuanfeng Wang; Mengting Fan; Mengyuan Zhang; Yao Zhou; Xiaoyan Cui; Dazhi Zhang; Ting Wang

doi:10.1039/D0CC05529H

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Far-red imaging of β-galactosidase through a phospha-fluorescein†

Zichao Ding,‡^a Chuanfeng Wang,‡^b Mengting Fan,^b Mengyuan Zhang,^b Yao Zhou,^b Xiaoyan Cui,

*^b Dazhi Zhang*^a and Ting Wang

*^a

Author affiliations

* Corresponding authors

^a College of Pharmacy, Second Military Medical University, Shanghai, P. R. China
E-mail: wangting1983927@gmail.com, zdzhang_yjhx@smmu.edu.cn

^b Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, P. R. China
E-mail: xycui@chem.ecnu.edu.cn

Abstract

The introduction of phosphine oxide into a fluorescein scaffold has yielded phospha-fluorescein with bathochromically shifted spectra, reliable photostability and solubility. Moreover, ratiometric and turn-on fluorescence in the decaging process has ensured that the phospha-fluorescein is a unique scaffold for fluorescence bioimaging. Probe DiMe-PF-Gal without further structural decoration was designed for accurately monitoring β-galactosidase in vivo.

Download options Please wait...

Supplementary files

Supplementary information PDF (1820K)

Article information

DOI: https://doi.org/10.1039/D0CC05529H
Article type: Communication
Submitted: 13 Aug 2020
Accepted: 04 Oct 2020
First published: 06 Oct 2020

Download Citation

Chem. Commun., 2020,56, 13579-13582

Permissions

Request permissions

Far-red imaging of β-galactosidase through a phospha-fluorescein

Z. Ding, C. Wang, M. Fan, M. Zhang, Y. Zhou, X. Cui, D. Zhang and T. Wang, Chem. Commun., 2020, 56, 13579 DOI: 10.1039/D0CC05529H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Chemical Communications

Far-red imaging of β-galactosidase through a phospha-fluorescein†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Far-red imaging of β-galactosidase through a phospha-fluorescein

Social activity

Search articles by author

Spotlight

Advertisements