Fluorescence assay for the determination of glutathione based on a ring-fused 2-pyridone derivative in dietary supplements

Wiktor Kasprzyk; Filip Koper; Agata Flis; Dominika Szreder; Elżbieta Pamuła; Dariusz Bogdał; Sławomir Wybraniec; Joanna Ortyl; Tomasz Świergosz

doi:10.1039/D0AN02245D

Fluorescence assay for the determination of glutathione based on a ring-fused 2-pyridone derivative in dietary supplements†

Wiktor Kasprzyk,

*^a Filip Koper,

^a Agata Flis,

^b Dominika Szreder,^a Elżbieta Pamuła,^b Dariusz Bogdał,^a Sławomir Wybraniec,

^c Joanna Ortyl

^a and Tomasz Świergosz

*^c

Author affiliations

* Corresponding authors

^a Department of Biotechnology and Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Kraków, Poland
E-mail: wiktor.kasprzyk@pk.edu.pl

^b Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Mickiewicza 30, Kraków, Poland

^c Department of Analytical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Kraków, Poland
E-mail: tomasz.swiergosz@pk.edu.pl

Abstract

Herein, a novel fluorescent method for the determination of GSH levels in aqueous solutions involving the utilization of citric acid as a derivatization reagent was developed. Therefore, the crucial parameters of the derivatization process were established from what has resulted in the development of a sensitive, reproducible, and accurate GSH assay. The method was validated, and its applicability in the characterization of the GSH concentration in dietary supplements concerning the selectivity in the determination of GSH over GSSG was both confirmed. The chemical structure of the new fluorophore 3-[(carboxymethyl)carbamoyl]-5-oxo-2,3-dihydro-5H-[1,3]thiazolo[3,2-a]pyridine-7-carboxylic acid – CTPC was elucidated using detailed NMR: one-dimensional (¹H, ¹³C), as well as two-dimensional NMR spectra (¹H–¹H COSY, ¹H–¹³C HSQC, ¹H–¹³C HMBC, ¹H–¹⁵N HSQC, ¹H–¹⁵N HMBC) experiments. Besides, the essential optical, biological and antioxidative properties of CTPC were investigated.

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DOI: https://doi.org/10.1039/D0AN02245D
Article type: Paper
Submitted: 18 Nov 2020
Accepted: 06 Jan 2021
First published: 08 Jan 2021

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Analyst, 2021,146, 1897-1906

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Fluorescence assay for the determination of glutathione based on a ring-fused 2-pyridone derivative in dietary supplements

W. Kasprzyk, F. Koper, A. Flis, D. Szreder, E. Pamuła, D. Bogdał, S. Wybraniec, J. Ortyl and T. Świergosz, Analyst, 2021, 146, 1897 DOI: 10.1039/D0AN02245D

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Fluorescence assay for the determination of glutathione based on a ring-fused 2-pyridone derivative in dietary supplements†

Abstract

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Fluorescence assay for the determination of glutathione based on a ring-fused 2-pyridone derivative in dietary supplements

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