Oily core/amphiphilic polymer shell nanocapsules change the intracellular fate of doxorubicin in breast cancer cells

Janaina Moreira Coelho; Nichollas Serafim Camargo; Rayane Ganassin; Márcia Cristina Oliveira Rocha; Carolin Merker; Julia Böttner; Irina Estrela-Lopis; Karen Rapp Py-Daniel; Katiúscia Vieira Jardim; Marcelo Henrique Sousa; Alicia Simalie Ombredane; Graziella Anselmo Joanitti; Renata Carvalho Silva; Ricardo Bentes Azevedo; João Paulo Figueiró Longo; Luis Alexandre Muehlmann

doi:10.1039/C9TB00587K

Oily core/amphiphilic polymer shell nanocapsules change the intracellular fate of doxorubicin in breast cancer cells

Janaina Moreira Coelho,^ab Nichollas Serafim Camargo,^ab Rayane Ganassin,^ab Márcia Cristina Oliveira Rocha,^b Carolin Merker,^c Julia Böttner,^c Irina Estrela-Lopis,^c Karen Rapp Py-Daniel,^b Katiúscia Vieira Jardim,^a Marcelo Henrique Sousa,

^a Alicia Simalie Ombredane,^abd Graziella Anselmo Joanitti,^abd Renata Carvalho Silva,^e Ricardo Bentes Azevedo,

^b João Paulo Figueiró Longo

^b and Luis Alexandre Muehlmann

*^a

Author affiliations

* Corresponding authors

^a Faculty of Ceilandia, University of Brasilia, Brasilia 72220-900, Brazil
E-mail: luisalex@unb.br

^b Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil

^c Institute of Medical Physics & Biophysics, Leipzig University, Leipzig 04107, Germany

^d Laboratory of Bioactive Compounds and Nanobiotechnology, Faculty of Ceilandia, University of Brasilia, Brasilia 72220-900, Brazil

^e Institute of Metrology, Quality and Technology (Inmetro), Rio de Janeiro, Brazil

Abstract

The aim of this work was to develop and test the in vitro biological activity of nanocapsules loaded with a doxorubicin (DOX) free base dissolved in a core of castor oil shelled by poly(methyl vinyl ether-co-maleic anhydride) conjugated to n-octadecylamine residues. This system was stable and monodisperse, with a hydrodynamic diameter of about 300 nm. These nanocapsules changed the intracellular distribution of DOX, from the nuclei to the cytoplasm, and exhibited higher toxicity towards cancer cells – 4T1 and MCF-7 – and significantly lower toxicity towards normal cells – NIH-3T3 and MCF-10A – in vitro. In conclusion, these nanocapsules are suitable DOX carriers, which remain to be studied in in vivo tumor models.

This article is part of the themed collections: Journal of Materials Chemistry B Emerging Investigators and Materials and Nano Research in Brazil

Journal of Materials Chemistry B

Oily core/amphiphilic polymer shell nanocapsules change the intracellular fate of doxorubicin in breast cancer cells

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Oily core/amphiphilic polymer shell nanocapsules change the intracellular fate of doxorubicin in breast cancer cells

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