Issue 32, 2019
From the journal:

Chemical Communications

Cytosolic delivery of CRISPR/Cas9 ribonucleoproteins for genome editing using chitosan-coated red fluorescent protein†

Jie Qiao, ORCID logo ‡ab   Wenli Sun,‡a   Siyu Lin,a   Rong Jin,c   Lixin Maabd  and  Yi Liu ORCID logo *abd  

* Corresponding authors

a State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, China
E-mail: yiliu0825@hubu.edu.cn

b Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, China

c Institute of Nanochemistry & Nanobiology, Shanghai University, Shanghai, China

d Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, School of Life Sciences, Hubei University, China

Abstract

Though plenty of viral and non-viral methods have been rapidly developed for the delivery of the CRISPR/Cas9 system, the direct delivery of Cas9 ribonucleoproteins (RNPs) into the nucleus for genome editing via the non-homologous end joining (NHEJ) or homology-directed repair (HDR) pathway, especially the latter one, remains a challenge. Here we report a delivery vehicle achieved by encapsulating red fluorescent protein (RFP) within chitosan (CS), which can simultaneously deliver engineered Cas9 RNPs with a poly-glutamate peptide tag (E-tag) and DNA donors into a range of cell types with high genome editing efficacy and non-cytotoxicity.

Graphical abstract: Cytosolic delivery of CRISPR/Cas9 ribonucleoproteins for genome editing using chitosan-coated red fluorescent protein

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Article information

DOI
https://doi.org/10.1039/C9CC00010K
Article type
Communication
Submitted
02 Jan 2019
Accepted
27 Mar 2019
First published
27 Mar 2019

Chem. Commun., 2019,55, 4707-4710

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Cytosolic delivery of CRISPR/Cas9 ribonucleoproteins for genome editing using chitosan-coated red fluorescent protein

J. Qiao, W. Sun, S. Lin, R. Jin, L. Ma and Y. Liu, Chem. Commun., 2019, 55, 4707 DOI: 10.1039/C9CC00010K

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