In vivo fate of free and encapsulated iron oxide nanoparticles after injection of labelled stem cells

Sumaira Ashraf; Arthur Taylor; Jack Sharkey; Michael Barrow; Patricia Murray; Bettina Wilm; Harish Poptani; Matthew J. Rosseinsky; Dave J. Adams; Raphaël Lévy

doi:10.1039/C8NA00098K

In vivo fate of free and encapsulated iron oxide nanoparticles after injection of labelled stem cells†

Sumaira Ashraf,

*^a Arthur Taylor,^bc Jack Sharkey,^bc Michael Barrow,

^d Patricia Murray,^bc Bettina Wilm,

^bc Harish Poptani,^bc Matthew J. Rosseinsky,^d Dave J. Adams

^de and Raphaël Lévy

*^a

Author affiliations

* Corresponding authors

^a Department of Biochemistry, Institute of Integrative Biology (IIB), University of Liverpool, Liverpool, UK
E-mail: rapha@liverpool.ac.uk, sumaira@liverpool.ac.uk

^b Department of Cellular and Molecular Physiology, Institute of Translational Medicine (ITM), University of Liverpool, Liverpool, UK

^c Centre for Preclinical Imaging, Institute of Translational Medicine (ITM), University of Liverpool, Liverpool, UK

^d Department of Chemistry, University of Liverpool, Liverpool, UK

^e School of Chemistry, University of Glasgow, Glasgow, UK

Abstract

Nanoparticle contrast agents are useful tools to label stem cells and monitor the in vivo bio-distribution of labeled cells in pre-clinical models of disease. In this context, understanding the in vivo fate of the particles after injection of labelled cells is important for their eventual clinical use as well as for the interpretation of imaging results. We examined how the formulation of superparamagnetic iron oxide nanoparticles (SPIONs) impacts the labelling efficiency, magnetic characteristics and fate of the particles by comparing individual SPIONs with polyelectrolyte multilayer capsules containing SPIONs. At low labelling concentration, encapsulated SPIONs served as an efficient labelling agent for stem cells. The bio-distribution after intra-cardiac injection of labelled cells was monitored longitudinally by MRI and as an endpoint by inductively coupled plasma-optical emission spectrometry. The results suggest that, after being released from labelled cells after cell death, both formulations of particles are initially stored in liver and spleen and are not completely cleared from these organs 2 weeks post-injection.

This article is part of the themed collections: International Year of the Periodic Table: Applications for magnetic materials and Nanoscale Advances Most Popular Articles so far

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In vivo fate of free and encapsulated iron oxide nanoparticles after injection of labelled stem cells†

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