Accurate detection of matrix metalloproteinase-2 activity in clinical gastric cancer tissues using a fluorescent probe

Fujuan Luan; Zuhong Yu; Ling Yin; Xia Leng; Yuxue Shi; Jie Wang; Haibin Shi; Weichang Chen

doi:10.1039/C8AY02789G

Accurate detection of matrix metalloproteinase-2 activity in clinical gastric cancer tissues using a fluorescent probe†

Fujuan Luan,‡^a Zuhong Yu,‡^a Ling Yin,^cd Xia Leng,^a Yuxue Shi,^a Jie Wang,^a Haibin Shi

*^b and Weichang Chen*^a

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* Corresponding authors

^a Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou 215006, P. R. China
E-mail: weichangchen@126.com

^b State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, P. R. China
E-mail: hbshi@suda.edu.cn

^c Key Laboratory of Organic Synthesis of Jiangsu Province, College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, P. R. China

^d Department of Chemistry and Chemical Engineering, Jining University, Qufu 273155, P. R. China

Abstract

Matrix metalloproteinase-2 (MMP-2) plays an important role in tumorigenesis and metastasis of gastric cancer. Therefore, specific and sensitive detection of MMP-2 is of great significance for clinical diagnosis and therapeutic evaluation of gastric cancer. Herein, we developed a new approach for accurate detection of MMP-2 activity both in gastric cancer cells and clinical tissues using a fluorescent probe Dab-PLGVRGY-FITC. The capability of the probe for detecting MMP-2 was systematically evaluated in gastric cancer MGC-803 cells and clinical gastric cancer tissues. Around 40-fold fluorescence enhancement at a wavelength of 520 nm was determined for the assays of the probe with recombinant MMP-2. The detection limit of MMP-2 was as low as 42 ng mL⁻¹. Strong fluorescence signals were detected in MGC-803 cells and mouse gastric cancer tissues. Fluorescence signal enhancements of 11 clinical gastric cancer tissue lysates were 5.8 ∼ 9 times (7.53 ± 0.77 on average) higher than those from gastritis patients. This probe could be potentially used for early diagnosis of gastric cancer in clinics.

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DOI: https://doi.org/10.1039/C8AY02789G
Article type: Paper
Submitted: 22 Dec 2018
Accepted: 11 Feb 2019
First published: 15 Feb 2019

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Anal. Methods, 2019,11, 1516-1521

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Accurate detection of matrix metalloproteinase-2 activity in clinical gastric cancer tissues using a fluorescent probe

F. Luan, Z. Yu, L. Yin, X. Leng, Y. Shi, J. Wang, H. Shi and W. Chen, Anal. Methods, 2019, 11, 1516 DOI: 10.1039/C8AY02789G

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Analytical Methods

Accurate detection of matrix metalloproteinase-2 activity in clinical gastric cancer tissues using a fluorescent probe†

Abstract

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Accurate detection of matrix metalloproteinase-2 activity in clinical gastric cancer tissues using a fluorescent probe

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