Epigenetic changes in cancer by Raman imaging, fluorescence imaging, AFM and scanning near-field optical microscopy (SNOM). Acetylation in normal and human cancer breast cells MCF10A, MCF7 and MDA-MB-231

Halina Abramczyk; Jakub Surmacki; Monika Kopeć; Alicja Klaudia Olejnik; Agnieszka Kaufman-Szymczyk; Krystyna Fabianowska-Majewska

doi:10.1039/C6AN00859C

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Epigenetic changes in cancer by Raman imaging, fluorescence imaging, AFM and scanning near-field optical microscopy (SNOM). Acetylation in normal and human cancer breast cells MCF10A, MCF7 and MDA-MB-231

Halina Abramczyk,*^a Jakub Surmacki,^a Monika Kopeć,^a Alicja Klaudia Olejnik,^b Agnieszka Kaufman-Szymczyk^c and Krystyna Fabianowska-Majewska^c

Author affiliations

* Corresponding authors

^a Institute of Applied Radiation Chemistry, Laboratory of Laser Molecular Spectroscopy, Lodz University of Technology, Lodz, Poland
E-mail: abramczy@mitr.p.lodz.pl

^b Institute of Applied Radiation Chemistry, Lodz University of Technology, Lodz, Poland

^c Department of Biomedical Chemistry, Medical University, Lodz, Poland

Abstract

This paper examines epigenetic changes in breast cancer by Raman imaging, fluorescence imaging, AFM and SNOM and discusses how they contribute to different aspects of tumourigenesis in malignant human breast epithelial cell lines MCF7 and MDA-MB-231 compared with non-malignant MCF10A cell lines. The paper focuses on information that can be extracted from Raman microscopy and Raman imaging for the biological material of nucleoli contained within the cell nucleus and lipid droplets within the cell cytoplasm. The biochemical composition of the nuclei and lipid droplets in the non-malignant and malignant human breast epithelial cell lines has been monitored. The potential of Raman microspectroscopy to monitor acetylation processes and a prognostic value of Raman biomarkers in breast cancer have been discussed.

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DOI: https://doi.org/10.1039/C6AN00859C
Article type: Paper
Submitted: 13 Apr 2016
Accepted: 19 Jul 2016
First published: 27 Jul 2016

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Analyst, 2016,141, 5646-5658

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Epigenetic changes in cancer by Raman imaging, fluorescence imaging, AFM and scanning near-field optical microscopy (SNOM). Acetylation in normal and human cancer breast cells MCF10A, MCF7 and MDA-MB-231

H. Abramczyk, J. Surmacki, M. Kopeć, A. K. Olejnik, A. Kaufman-Szymczyk and K. Fabianowska-Majewska, Analyst, 2016, 141, 5646 DOI: 10.1039/C6AN00859C

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Epigenetic changes in cancer by Raman imaging, fluorescence imaging, AFM and scanning near-field optical microscopy (SNOM). Acetylation in normal and human cancer breast cells MCF10A, MCF7 and MDA-MB-231

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Epigenetic changes in cancer by Raman imaging, fluorescence imaging, AFM and scanning near-field optical microscopy (SNOM). Acetylation in normal and human cancer breast cells MCF10A, MCF7 and MDA-MB-231

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