Synthesis of highly fluorescent P,O-g-C3N4 nanodots for the label-free detection of Cu2+ and acetylcholinesterase activity†
Abstract
Highly fluorescent phosphorus, oxygen-doped graphitic carbon nitride nanodots (P,O-g-C3N4 nanodots) were synthesized using chemical oxidation and hydrothermal etching of bulk P-g-C3N4 obtained via pyrolysis of phytic acid and melamine. The P,O-g-C3N4 nanodots emitted strong blue fluorescence with a high quantum yield of 90.2%, and displayed high resistance to photobleaching and high ionic strength. A sensitive and facile fluorescence sensing approach for Cu2+ was developed through fluorescence quenching based on the static fluorescence quenching and photoinduced electron transfer. Under optimal conditions, a rapid detection of Cu2+ could be completed in 5 min with a detection limit of 2 nM, and a linearity ranging from 0 to 1 μM. Using acetylthiocholine (ATCh) as the substrate, the fluorescence of the P,O-g-C3N4 nanodots–Cu2+ system could be sensitively turned on in the presence of acetylcholinesterase (AChE) through the reaction between Cu2+ and thiocholine, the hydrolysis product of ATCh by AChE. A linearity ranging from 0.01 to 3 mU mL−1 could be obtained with a detection limit of 0.01 mU mL−1. In addition, the proposed approach showed potential application for the detection of Cu2+ in natural water samples and AChE activity in human plasma.
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