Synthesis and characterization of bioreducible heparin-polyethyleneimine nanogels: application as imaging-guided photosensitizer delivery vehicle in photodynamic therapy

Tilahun Ayane Debele; Shewaye Lakew Mekuria; Shuian-Yin Lin; Hsieh-Chih Tsai

doi:10.1039/C5RA25650J

Synthesis and characterization of bioreducible heparin-polyethyleneimine nanogels: application as imaging-guided photosensitizer delivery vehicle in photodynamic therapy

Tilahun Ayane Debele,^a Shewaye Lakew Mekuria,^a Shuian-Yin Lin^b and Hsieh-Chih Tsai*^a

* Corresponding authors

^a Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan, Republic of China
E-mail: h.c.tsai@mail.ntust.edu.tw
Fax: +886-2-27303733
Tel: +886-2-27303625

^b National Applied Research Laboratories, Instrument Technology Research Center, Hsinchu 300, Taiwan, Republic of China

Abstract

Herein, we synthesized and characterized novel bioreducible heparin polyethyleneimine (HPC) nanogels consisting of heparin, branched polyethyleneimine (PEI) and L-cysteine. ¹H-NMR and FTIR analysis confirmed the formation of HPC nanogels while TEM and dynamic light scattering revealed uniform spherical nanoparticles with average diameter of <200 nm. Zinc phthalocyanine (ZnPC) was encapsulated via the dialysis method and the drug is released in vitro from disulfide-containing HPC nanogels in a redox-sensitive manner at low pH. Additionally, HPC nanogels possess bright blue fluorescence which eliminates the use of additional probing agent in image-guided drug delivery. Moreover, singlet oxygen detection revealed that nanogels prevented ZnPc aggregation thus enhancing ¹O₂ generation and photodynamic therapy (PDT) efficacy. These results showed that disulfide crosslinked HPC nanogels are promising vehicles for stimulated photosensitizer delivery in advanced PDT.

Article information

https://doi.org/10.1039/C5RA25650J

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Paper

Submitted

02 Dec 2015

Accepted

19 Jan 2016

First published

22 Jan 2016

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RSC Adv., 2016,6, 14692-14704

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Synthesis and characterization of bioreducible heparin-polyethyleneimine nanogels: application as imaging-guided photosensitizer delivery vehicle in photodynamic therapy

T. A. Debele, S. L. Mekuria, S. Lin and H. Tsai, RSC Adv., 2016, 6, 14692 DOI: 10.1039/C5RA25650J

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Synthesis and characterization of bioreducible heparin-polyethyleneimine nanogels: application as imaging-guided photosensitizer delivery vehicle in photodynamic therapy

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