Issue 32, 2015
From the journal:

Organic & Biomolecular Chemistry

A simple fluorescent probe based on a pyrene derivative for rapid detection of protamine and monitoring of trypsin activity†

Baiyang Tang,‡ab   Yan Yang,‡a   Gefu Wang,c   Zhiyi Yao,*a   Li Zhang*b  and  Hai-Chen Wu ORCID logo *a  

* Corresponding authors

a Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China
E-mail: yaozy@ihep.ac.cn, haichenwu@ihep.ac.cn
Fax: +86 10 88235745
Tel: +86 10 88235745

b School of Materials Science and Engineering, Zhengzhou University, Zhengzhou 450052, China
E-mail: lizhang9@zzu.edu.cn

c School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China

Abstract

We report the synthesis of a simple pyrene derivative and its application in protamine detection and monitoring of trypsin activity. This assay can be conducted in aqueous solution and features rapid response, visual detection, high sensitivity and selectivity. The limit of detection of protamine was 0.5 μg mL−1. The IC50 value of a soybean trypsin inhibitor was estimated to be 0.51 U mL−1.

Graphical abstract: A simple fluorescent probe based on a pyrene derivative for rapid detection of protamine and monitoring of trypsin activity

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Supplementary files

Article information

DOI
https://doi.org/10.1039/C5OB01034A
Article type
Paper
Submitted
22 May 2015
Accepted
09 Jul 2015
First published
09 Jul 2015

Org. Biomol. Chem., 2015,13, 8708-8712

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A simple fluorescent probe based on a pyrene derivative for rapid detection of protamine and monitoring of trypsin activity

B. Tang, Y. Yang, G. Wang, Z. Yao, L. Zhang and H. Wu, Org. Biomol. Chem., 2015, 13, 8708 DOI: 10.1039/C5OB01034A

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