Issue 25, 2015
From the journal:

Organic & Biomolecular Chemistry

Synthesis of DNA fragments containing 2′-deoxy-4′-selenonucleoside units using DNA polymerases: comparison of dNTPs with O, S and Se at the 4′-position in replication†

N. Tarashima,a   T. Sumitomo,a   H. Ando,a   K. Furukawa,a   T. Ishidaa  and  N. Minakawa*a  

* Corresponding authors

a Graduate School of Pharmaceutical Sciences, Tokushima University, Shomachi 1-78-1, Tokushima 770-8505, Japan
E-mail: minakawa@tokushima-u.ac.jp
Fax: +81 (0)88-633-7288
Tel: +81 (0)88-633-7288

Abstract

4′-SelenoDNA fragments were synthesized for the first time using 4′-selenothymidine triphosphate (SeTTP) by taking advantage of its bioequivalence against DNA polymerases. DNA fragments each with a homologous element (O, S or Se) at the 4′-position of the thymidine units were effectively amplified using KOD Dash DNA polymerase.

Graphical abstract: Synthesis of DNA fragments containing 2′-deoxy-4′-selenonucleoside units using DNA polymerases: comparison of dNTPs with O, S and Se at the 4′-position in replication

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Article information

DOI
https://doi.org/10.1039/C5OB00941C
Article type
Communication
Submitted
09 May 2015
Accepted
20 May 2015
First published
20 May 2015

Org. Biomol. Chem., 2015,13, 6949-6952

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Synthesis of DNA fragments containing 2′-deoxy-4′-selenonucleoside units using DNA polymerases: comparison of dNTPs with O, S and Se at the 4′-position in replication

N. Tarashima, T. Sumitomo, H. Ando, K. Furukawa, T. Ishida and N. Minakawa, Org. Biomol. Chem., 2015, 13, 6949 DOI: 10.1039/C5OB00941C

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