Issue 2, 2015
From the journal:

Molecular BioSystems

NMR analysis of budding yeast metabolomics: a rapid method for sample preparation†

C. Airoldi,‡*ab   F. Tripodi,‡ab   C. Guzzi,ab   R. Nicastroab  and  P. Coccetti*ab  

* Corresponding authors

a Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy
E-mail: cristina.airoldi@unimib.it, paola.coccetti@unimib.it

b SYSBIO, Centre of Systems Biology, P.zza della Scienza 2, Italy

Abstract

Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by 1H-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.

Graphical abstract: NMR analysis of budding yeast metabolomics: a rapid method for sample preparation

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Article information

DOI
https://doi.org/10.1039/C4MB00452C
Article type
Paper
Submitted
31 Jul 2014
Accepted
13 Oct 2014
First published
15 Oct 2014

Mol. BioSyst., 2015,11, 379-383

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NMR analysis of budding yeast metabolomics: a rapid method for sample preparation

C. Airoldi, F. Tripodi, C. Guzzi, R. Nicastro and P. Coccetti, Mol. BioSyst., 2015, 11, 379 DOI: 10.1039/C4MB00452C

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