Issue 7, 2014
From the journal:

Molecular BioSystems

Effects of Tet-induced oxidation products of 5-methylcytosine on Dnmt1- and DNMT3a-mediated cytosine methylation†

Debin Ji,a   Krystal Lin,b   Jikui Songb  and  Yinsheng Wang*a  

* Corresponding authors

a Department of Chemistry, University of California, Riverside, CA 92521, USA
E-mail: yinsheng.wang@ucr.edu
Tel: +1 951 8272700

b Department of Biochemistry, University of California, Riverside, CA 92521, USA

Abstract

We investigated systematically the effects of Tet-induced oxidation products of 5-methylcytosine on Dnmt1- and DNMT3a-mediated cytosine methylation in synthetic duplex DNA. We found that the replacement of 5-methylcytosine at a CpG site with a 5-hydroxymethylcytosine, 5-formylcytosine, 5-carboxylcytosine or 5-hydroxymethyluracil resulted in altered methylation of cytosine at both the opposite and the neighboring CpG sites. Our results provided important new knowledge about the implications of the 5-methylcytosine oxidation products in maintenance cytosine methylation.

Graphical abstract: Effects of Tet-induced oxidation products of 5-methylcytosine on Dnmt1- and DNMT3a-mediated cytosine methylation

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Article information

DOI
https://doi.org/10.1039/C4MB00150H
Article type
Communication
Submitted
11 Mar 2014
Accepted
13 Apr 2014
First published
14 Apr 2014

Mol. BioSyst., 2014,10, 1749-1752

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Effects of Tet-induced oxidation products of 5-methylcytosine on Dnmt1- and DNMT3a-mediated cytosine methylation

D. Ji, K. Lin, J. Song and Y. Wang, Mol. BioSyst., 2014, 10, 1749 DOI: 10.1039/C4MB00150H

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