Issue 24, 2013
From the journal:

Journal of Materials Chemistry B

Folate-conjugated cross-linked magnetic nanoparticles as potential magnetic resonance probes for in vivo cancer imaging†

Hee-Man Yang,‡a   Chan Woo Park,‡b   Pan Kee Bae,c   Taebin Ahn,b   Bum-Kyoung Seo,a   Bong Hyun Chungc  and  Jong-Duk Kim*b  

* Corresponding authors

a Decontamination & Decommissioning Research Division, Korea Atomic Energy Research Institute, Daedeok-daero 989-111, Yuseong-gu, Daejeon 305-353, Republic of Korea
E-mail: hmyang@kaeri.re.kr
Fax: +82-42-868-8667
Tel: +82-42-868-4537

b Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-roYuseong-gu, Daejeon 305-701, Republic of korea
E-mail: kjd@kaist.ac.kr
Fax: +82-42-350-3910
Tel: +82-42-350-3961

c BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea
Fax: +82-42-861-1759
Tel: +82-42-860-4442

Abstract

Superparamagnetic iron oxide nanoparticles are widely used as nanoprobes for magnetic resonance imaging (MRI). In this study, a novel type of cross-linked magnetic nanoparticle was developed in an effort to improve the structural stability of amphiphilic polymer-coated iron oxide nanoparticles. Iron oxide nanocrystals were coated with a cross-linkable amphiphilic graft copolymer, poly(succinimide) grafted with folate-conjugated polyethylene glycol (PEG) and alkyl chains. The tumor-specific targeting ligand, folate, was included to target and detect cancer cells. The hydrophobic portions of the amphiphilic copolymer on the surfaces of the nanoparticles were cross-linked via an aminolysis reaction between the succinimide units and a bifunctional primary amine. The folate-conjugated cross-linked magnetic nanoparticles (F-CLMNPs) were 40 nm in diameter and displayed a low cytotoxicity, even at relatively high concentrations. The F-CLMNPs exhibited highly efficient intracellular uptake into KB cells, which overexpress the folate receptor, as determined by fluorescence microscopy, flow cytometry, and Prussian blue staining. In vivo MR images of a mouse bearing a KB cell tumor displayed a 75% drop in the T2 signal in the tumor tissues within 3 hours. These results indicated that the F-CLMNPs accumulated at the tumor site and were highly effective for tumor detection using in vivo MRI techniques.

Graphical abstract: Folate-conjugated cross-linked magnetic nanoparticles as potential magnetic resonance probes for in vivo cancer imaging

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Article information

DOI
https://doi.org/10.1039/C3TB20295J
Article type
Paper
Submitted
04 Mar 2013
Accepted
16 Apr 2013
First published
17 Apr 2013

J. Mater. Chem. B, 2013,1, 3035-3043

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Folate-conjugated cross-linked magnetic nanoparticles as potential magnetic resonance probes for in vivo cancer imaging

H. Yang, C. W. Park, P. K. Bae, T. Ahn, B. Seo, B. H. Chung and J. Kim, J. Mater. Chem. B, 2013, 1, 3035 DOI: 10.1039/C3TB20295J

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