Issue 7, 2013
From the journal:

Lab on a Chip

SU-8 based microprobes for simultaneous neural depth recording and drug delivery in the brain

Ane Altuna,†*a   Elisa Bellistri,†b   Elena Cid,b   Paloma Aivar,b   Beatriz Gal,bc   Javier Berganzo,a   Gemma Gabriel,de   Anton Guimerà,de   Rosa Villa,de   Luis J. Fernández‡ef  and  Liset Menendez de la Prida‡*b  

* Corresponding authors

a MEMS/MST Department, Ikerlan S. Coop., Mondragón, Spain
E-mail: ane.altuna@ikerlan.es

b Instituto Cajal, CSIC, Madrid 28002, Spain
E-mail: lmprida@cajal.csic.es

c Universidad Europea de Madrid, Madrid 28670, Spain

d Instituto de Microelectrónica de Barcelona (IMB-CNM), CSIC, Campus UAB, 08913 Bellaterra, Barcelona, Spain

e Centro de Investigación Biomédica en Red, Biomateriales y Nanomedicina (CIBER-BBN), Spain

f Aragón Institute of Engineering Research (I3A), Group of Structural Mechanics and Materials Modelling (GEMM), Universidad de Zaragoza, Spain

Abstract

While novel influential concepts in neuroscience bring the focus to local activities generated within a few tens of cubic micrometers in the brain, we are still devoid of appropriate tools to record and manipulate pharmacologically neuronal activity at this fine scale. Here we designed, fabricated and encapsulated microprobes for simultaneous depth recording and drug delivery using exclusively the polymer SU-8 as structural material. A tetrode- and linear-like electrode patterning was combined for the first time with single and double fluidic microchannels for independent drug delivery. The device was tested experimentally using the in vivo anesthetized rat preparation. Both probe types successfully recorded detailed spatiotemporal features of local field potentials and single-cell activity at a resolution never attained before with integrated fluidic probes. Drug delivery was achieved with high spatial and temporal precision in a range from tens of nanoliters to a few microliters, as confirmed histologically. These technological advancements will foster a wide range of neural applications aimed at simultaneous monitoring of brain activity and delivery at a very precise micrometer scale.

Graphical abstract: SU-8 based microprobes for simultaneous neural depth recording and drug delivery in the brain

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Article information

DOI
https://doi.org/10.1039/C3LC41364K
Article type
Paper
Submitted
12 Dec 2012
Accepted
18 Jan 2013
First published
21 Jan 2013

Lab Chip, 2013,13, 1422-1430

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SU-8 based microprobes for simultaneous neural depth recording and drug delivery in the brain

A. Altuna, E. Bellistri, E. Cid, P. Aivar, B. Gal, J. Berganzo, G. Gabriel, A. Guimerà, R. Villa, L. J. Fernández and L. Menendez de la Prida, Lab Chip, 2013, 13, 1422 DOI: 10.1039/C3LC41364K

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