Issue 9, 2013
From the journal:

Lab on a Chip

Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples†

A. Ahmad Tajudin,ah   K. Petersson,a   A. Lenshof,a   A.-M. Swärd-Nilsson,c   L. Åberg,c   G. Marko-Varga,a   J. Malm,b   H. Liljabdei  and  T. Laurell*afg  

* Corresponding authors

a Department of Measurement Technology and Industrial Electrical Engineering, Lund University, Box 118, Lund, Sweden

b Department of Laboratory Medicine, Division of Clinical Chemistry, Skåne University Hospital (SUS), 20502, Malmö, Sweden

c Clinical Immunology and Transfusion Medicine, University and Regional Laboratories, Region Skåne, Lund, Sweden

d Department of Laboratory Medicine, Surgery (Urology), and Medicine (GU-Oncology), Memorial Sloan-Kettering Cancer Center, New York, NY10065, USA

e Institute of Biomedical Technology, University of Tampere, Finland

f Department of Biomedical Engineering, Dongguk University, Seoul, Korea

g CREATE Health, BMC D13, Lund University, Lund, Sweden

h Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

i Nuffield Department of Surgical Sciences, University of Oxford, Oxford, United Kingdom

Abstract

On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38–40%) rate was 50 μl min−1 giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7–100 ng ml−1 within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%.

Graphical abstract: Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples

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Article information

DOI
https://doi.org/10.1039/C3LC41269E
Article type
Paper
Submitted
17 Nov 2012
Accepted
27 Feb 2013
First published
27 Feb 2013

Lab Chip, 2013,13, 1790-1796

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Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples

A. A. Tajudin, K. Petersson, A. Lenshof, A.-M. Swärd-Nilsson, L. Åberg, G. Marko-Varga, J. Malm, H. Lilja and T. Laurell, Lab Chip, 2013, 13, 1790 DOI: 10.1039/C3LC41269E

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