An electrorotation technique for measuring the dielectric properties of cells with simultaneous use of negative quadrupolar dielectrophoresis and electrorotation

Song-I Han; Young-Don Joo; Ki-Ho Han

doi:10.1039/C3AN36261B

An electrorotation technique for measuring the dielectric properties of cells with simultaneous use of negative quadrupolar dielectrophoresis and electrorotation†

Song-I Han,^a Young-Don Joo^b and Ki-Ho Han*^a

* Corresponding authors

^a Department of Nano Engineering, Center for Nano Manufacturing, Inje University, 607 Obang-dong, Gimhae 621-749, Republic of Korea
E-mail: mems@inje.ac.kr
Fax: +82-55-320-3631
Tel: +82-55-320-3715

^b Department of Hemato-Oncology, Haeundae Paik Hospital, Inje University, Busan 612-030, Republic of Korea

Abstract

This paper presents an effective electrorotation technique for measuring the dielectric properties of cells using a superposed electrical signal, which can simultaneously generate negative quadrupolar dielectrophoretic (nQDEP) force and electrorotational (ROT) torque. The proposed technique involves a three-dimensional (3D) octode, which includes four electrodes arranged in a crisscross pattern on the top and bottom of a microchannel, respectively. A single cell was trapped in the center of the 3D octode by the nQDEP force and simultaneously rotated by the ROT torque. Using the proposed electrorotation technique, ROT spectra of human leukocyte subpopulations (T and B lymphocytes, granulocytes, and monocytes) and metastatic human breast (SkBr3) and lung (A549) cancer cell lines were accurately measured without any disturbance. Torque on the cells generated by the ROT signal was analyzed theoretically based on the single-shell dielectric model for the cells. Furthermore, the dielectric properties of the cells, such as area-specific membrane capacitance and cytoplasm conductivity, were extracted using the measured ROT spectra and the analyzed torque.

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DOI: https://doi.org/10.1039/C3AN36261B
Article type: Paper
Submitted: 06 Sep 2012
Accepted: 16 Jan 2013
First published: 17 Jan 2013

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Analyst, 2013,138, 1529-1537

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An electrorotation technique for measuring the dielectric properties of cells with simultaneous use of negative quadrupolar dielectrophoresis and electrorotation

S. Han, Y. Joo and K. Han, Analyst, 2013, 138, 1529 DOI: 10.1039/C3AN36261B

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An electrorotation technique for measuring the dielectric properties of cells with simultaneous use of negative quadrupolar dielectrophoresis and electrorotation†

Abstract

Supplementary files

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An electrorotation technique for measuring the dielectric properties of cells with simultaneous use of negative quadrupolar dielectrophoresis and electrorotation

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